| pubmed-article:11205271 | rdf:type | pubmed:Citation | lld:pubmed |
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| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0015780 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0007138 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0005682 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0015127 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C1314792 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C1996904 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C1880497 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0078437 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C0130379 | lld:lifeskim |
| pubmed-article:11205271 | lifeskim:mentions | umls-concept:C1512523 | lld:lifeskim |
| pubmed-article:11205271 | pubmed:issue | 6B | lld:pubmed |
| pubmed-article:11205271 | pubmed:dateCreated | 2001-2-6 | lld:pubmed |
| pubmed-article:11205271 | pubmed:abstractText | The effect of N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride) (W-7), a widely used calmodulin inhibitor, on intracellular free Ca2+ levels ([Ca2+]i) in bladder female transitional cancer (BFTC) cells was examined using fura-2 as a Ca2+ dye. W-7 (10-1000 microM) caused an increase in [Ca2+]i in a concentration-dependent manner with an EC50 of 75 microM. The [Ca2+]i response was composed of an initial rise and a sustained plateau without significant decaying during the measurement of 250 seconds. Extracellular Ca2+ removal dramatically decreased the Ca2+ signals by 50-90%. W-7 (100 microM) failed to induce a [Ca2+]i increase in Ca(2+)-free medium after pretreatment with thapsigargin (1 microM), an endoplasmic reticulum Ca2+ pump inhibitor; conversely, W-7 pretreatment abolished the Ca2+ release induced by thapsigargin. Addition of 3 mM Ca2+ increased [Ca2+]i after preincubation with 100 microM W-7 in Ca(2+)-free medium. W-7-induced Ca2+ release was not altered by inhibiting phospholipase C with 2 microM 1-(6-((17b-3-methoxyestra-1,3,5 (10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione) (U73122). Together, this study shows that W-7 caused [Ca2+]i increases in human bladder cancer cells by releasing intracellular Ca2+ from the endoplasmic reticulum and also by causing extracellular Ca2+ influx with the later playing a dominant role. The W-7-induced intracellular Ca2+ release was uncoupled to a prior elevation in intracellular levels of inositol 1,4,5-trisphosphate (IP3). | lld:pubmed |
| pubmed-article:11205271 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11205271 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11205271 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11205271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11205271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11205271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11205271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11205271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11205271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11205271 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11205271 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11205271 | pubmed:issn | 0250-7005 | lld:pubmed |
| pubmed-article:11205271 | pubmed:author | pubmed-author:YuC CCC | lld:pubmed |
| pubmed-article:11205271 | pubmed:author | pubmed-author:HuangJ KJK | lld:pubmed |
| pubmed-article:11205271 | pubmed:author | pubmed-author:JanC RCR | lld:pubmed |
| pubmed-article:11205271 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11205271 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:11205271 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11205271 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11205271 | pubmed:pagination | 4355-9 | lld:pubmed |
| pubmed-article:11205271 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:11205271 | pubmed:articleTitle | N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride) (W-7) causes increases in intracellular free Ca2+ levels in bladder female transitional carcinoma (BFTC) cells. | lld:pubmed |
| pubmed-article:11205271 | pubmed:affiliation | Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Taiwan 813. crjan@isca.vghks.gov.tw | lld:pubmed |
| pubmed-article:11205271 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11205271 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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