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rdf:type |
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lifeskim:mentions |
umls-concept:C0005682,
umls-concept:C0007138,
umls-concept:C0007634,
umls-concept:C0015127,
umls-concept:C0015780,
umls-concept:C0078437,
umls-concept:C0130379,
umls-concept:C0178719,
umls-concept:C0441889,
umls-concept:C0442805,
umls-concept:C0596235,
umls-concept:C1314792,
umls-concept:C1512523,
umls-concept:C1880497,
umls-concept:C1996904
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pubmed:issue |
6B
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pubmed:dateCreated |
2001-2-6
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pubmed:abstractText |
The effect of N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride) (W-7), a widely used calmodulin inhibitor, on intracellular free Ca2+ levels ([Ca2+]i) in bladder female transitional cancer (BFTC) cells was examined using fura-2 as a Ca2+ dye. W-7 (10-1000 microM) caused an increase in [Ca2+]i in a concentration-dependent manner with an EC50 of 75 microM. The [Ca2+]i response was composed of an initial rise and a sustained plateau without significant decaying during the measurement of 250 seconds. Extracellular Ca2+ removal dramatically decreased the Ca2+ signals by 50-90%. W-7 (100 microM) failed to induce a [Ca2+]i increase in Ca(2+)-free medium after pretreatment with thapsigargin (1 microM), an endoplasmic reticulum Ca2+ pump inhibitor; conversely, W-7 pretreatment abolished the Ca2+ release induced by thapsigargin. Addition of 3 mM Ca2+ increased [Ca2+]i after preincubation with 100 microM W-7 in Ca(2+)-free medium. W-7-induced Ca2+ release was not altered by inhibiting phospholipase C with 2 microM 1-(6-((17b-3-methoxyestra-1,3,5 (10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione) (U73122). Together, this study shows that W-7 caused [Ca2+]i increases in human bladder cancer cells by releasing intracellular Ca2+ from the endoplasmic reticulum and also by causing extracellular Ca2+ influx with the later playing a dominant role. The W-7-induced intracellular Ca2+ release was uncoupled to a prior elevation in intracellular levels of inositol 1,4,5-trisphosphate (IP3).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Estrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/W 7
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pubmed:status |
MEDLINE
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pubmed:issn |
0250-7005
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4355-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11205271-Adenosine Triphosphate,
pubmed-meshheading:11205271-Calcium,
pubmed-meshheading:11205271-Calcium Signaling,
pubmed-meshheading:11205271-Carcinoma, Transitional Cell,
pubmed-meshheading:11205271-Culture Media,
pubmed-meshheading:11205271-Dose-Response Relationship, Drug,
pubmed-meshheading:11205271-Enzyme Inhibitors,
pubmed-meshheading:11205271-Estrenes,
pubmed-meshheading:11205271-Female,
pubmed-meshheading:11205271-Fluorescent Dyes,
pubmed-meshheading:11205271-Fura-2,
pubmed-meshheading:11205271-Humans,
pubmed-meshheading:11205271-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:11205271-Pyrrolidinones,
pubmed-meshheading:11205271-Sulfonamides,
pubmed-meshheading:11205271-Thapsigargin,
pubmed-meshheading:11205271-Tumor Cells, Cultured,
pubmed-meshheading:11205271-Type C Phospholipases,
pubmed-meshheading:11205271-Urinary Bladder Neoplasms
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pubmed:articleTitle |
N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride) (W-7) causes increases in intracellular free Ca2+ levels in bladder female transitional carcinoma (BFTC) cells.
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pubmed:affiliation |
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Taiwan 813. crjan@isca.vghks.gov.tw
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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