Source:http://linkedlifedata.com/resource/pubmed/id/11200253
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-1-25
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| pubmed:abstractText |
The effects of lecithinized superoxide dismutase (PC-SOD) and/or methylpredisolone (MP) in preventing secondary pathological changes after spinal cord injury (SCI) were investigated in rats with reference to recovery of hindlimb motor function and expression of mRNA of pro-inflammatory and neurotrophic genes. Hindlimb motor function was assessed as the BBB open field locomotor scores. The BBB scores of three groups treated with either PC-SOD (40,000 units/kg), MP (30 mg/kg), or a combination of PC-SOD and MP (PC-SOD+MP) increased with time until 3 days after SCI, and were significantly higher than that of the control group (p < 0.05). Thereafter, the score of the PC-SOD group increased, whereas that of the MP group showed a temporary decrease from day 3 to 5 and then it gradually recovered. The scores in all groups reached a plateau about 18 days after SCI. The PC-SOD+MP group did not show a synergism but a tendency similar to that of the MP group. PC-SOD and MP had down-regulatory effects on mRNA expression of pro-inflammatory substances such as interleukin-1beta (IL-1beta), intercellular adhesion molecule-1 (ICAM-1), and inducible-nitric oxide synthetase (i-NOS) after spinal cord compression at 3, 6, and 24 h, respectively, as judged by a semiquantitative reverse transcription-polymerase chain reaction and on the lipid peroxide (LPO) level 1 h after injury as determined by thiobarbituric acid-reactive substances. The suppression of pro-inflammatory genes expression, especially IL-1beta were greater in the MP group than in the PC-SOD group, while suppression of LPO level was similar in these two groups. PC-SOD+MP treatment augmented the suppression of all three pro-inflammatory genes expression and the decrease of the LPO level. The level of neurotrophin-3 (NT-3) mRNA increased from 6 h after SCI and reached a maximum after 48 h. NT-3 mRNA level was enhanced by PC-SOD treatment, but not by MP treatment. Thus, the effect of MP in suppressing these pro-inflammatory genes expression was more than that of PC-SOD. The difference in motor function in the early and later stage may be partially due to differences in expression of IL-1beta and NT-3 after either treatment, through an IL-1beta-dependent or NT-3-mediated repair response.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol-3-Phosphate Dehydrogenase...,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerolphosphate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Methylprednisolone,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotrophin 3,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase,
http://linkedlifedata.com/resource/pubmed/chemical/Thiobarbituric Acid Reactive...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0897-7151
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
18
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
93-103
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11200253-Animals,
pubmed-meshheading:11200253-Gene Expression Regulation,
pubmed-meshheading:11200253-Glycerol-3-Phosphate Dehydrogenase (NAD+),
pubmed-meshheading:11200253-Glycerolphosphate Dehydrogenase,
pubmed-meshheading:11200253-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11200253-Interleukin-1,
pubmed-meshheading:11200253-Male,
pubmed-meshheading:11200253-Methylprednisolone,
pubmed-meshheading:11200253-Movement Disorders,
pubmed-meshheading:11200253-Myelitis,
pubmed-meshheading:11200253-Nerve Growth Factors,
pubmed-meshheading:11200253-Neurotrophin 3,
pubmed-meshheading:11200253-Nitric Oxide Synthase,
pubmed-meshheading:11200253-Phosphatidylcholines,
pubmed-meshheading:11200253-RNA, Messenger,
pubmed-meshheading:11200253-Rats,
pubmed-meshheading:11200253-Rats, Wistar,
pubmed-meshheading:11200253-Recovery of Function,
pubmed-meshheading:11200253-Spinal Cord Injuries,
pubmed-meshheading:11200253-Superoxide Dismutase,
pubmed-meshheading:11200253-Thiobarbituric Acid Reactive Substances,
pubmed-meshheading:11200253-Transcription, Genetic
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| pubmed:year |
2001
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| pubmed:articleTitle |
Preventive effects of lecithinized superoxide dismutase and methylprednisolone on spinal cord injury in rats: transcriptional regulation of inflammatory and neurotrophic genes.
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| pubmed:affiliation |
Department of Orthopedic Surgery, School of Medicine, the University of Tokushima, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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