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rdf:type |
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lifeskim:mentions |
umls-concept:C0013879,
umls-concept:C0038164,
umls-concept:C0040649,
umls-concept:C0043393,
umls-concept:C0056695,
umls-concept:C0079419,
umls-concept:C0086418,
umls-concept:C0242210,
umls-concept:C0439596,
umls-concept:C0441655,
umls-concept:C1167622,
umls-concept:C1333336,
umls-concept:C1334043
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pubmed:issue |
1
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pubmed:dateCreated |
2001-1-19
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pubmed:abstractText |
The tumor suppressor protein p53 regulates various cellular responses to DNA damage and plays a significant role in DNA repair. The nuclear p300/cyclic AMP-responsive element binding (CREB)-binding protein (CBP) proteins act as coactivators in supporting the transcription function of p53. We examined the role of the human homologue of yeast Rad23 protein A (hHR23A), one of the two human homologues of the Saccharomyces cerevisiae nucleotide excision repair gene product Rad23, in the p300/CBP-associated regulation of p53 activity. Overexpression of wild-type hHR23A inhibits the p53 transcriptional activity and results in a decreased steady-state protein level of cellular p53. The inhibitory effect of hHR23A can be overcome by the concomitant expression of p300, CBP, and p300 segments harboring C/H1 domain and neutralized by the coexpression of HIV accessory protein Vpr, which binds COOH terminus of hHR23A/B. Additionally, hHR23A was shown to interact in vitro and in vivo with p300 segments harboring C/H1 domain. These studies provide evidence for the involvement of hHR23A in the regulation of p53 activity through p300/CBP. Although the precise direct role of hHR23 proteins in regulation of p53 and DNA repair remains to be elucidated, our data suggest that the interaction between hHR23A and p300/CBP has important implications in cross-talk between the p53 pathway and DNA repair.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Repair Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E1A-Associated p300 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Ep300 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/MDM2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Mdm2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-mdm2,
http://linkedlifedata.com/resource/pubmed/chemical/RAD23A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
64-70
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11196199-Animals,
pubmed-meshheading:11196199-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:11196199-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:11196199-Cyclins,
pubmed-meshheading:11196199-DNA Repair Enzymes,
pubmed-meshheading:11196199-DNA-Binding Proteins,
pubmed-meshheading:11196199-Down-Regulation,
pubmed-meshheading:11196199-E1A-Associated p300 Protein,
pubmed-meshheading:11196199-Gene Expression Regulation,
pubmed-meshheading:11196199-Humans,
pubmed-meshheading:11196199-Mice,
pubmed-meshheading:11196199-Nuclear Proteins,
pubmed-meshheading:11196199-Protein Binding,
pubmed-meshheading:11196199-Protein Structure, Tertiary,
pubmed-meshheading:11196199-Proto-Oncogene Proteins,
pubmed-meshheading:11196199-Proto-Oncogene Proteins c-mdm2,
pubmed-meshheading:11196199-Trans-Activators,
pubmed-meshheading:11196199-Transcription, Genetic,
pubmed-meshheading:11196199-Transcriptional Activation,
pubmed-meshheading:11196199-Tumor Suppressor Protein p53
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pubmed:year |
2001
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pubmed:articleTitle |
Human homologue of yeast Rad23 protein A interacts with p300/cyclic AMP-responsive element binding (CREB)-binding protein to down-regulate transcriptional activity of p53.
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pubmed:affiliation |
Department of Radiology Ohio State University, Columbus 43210, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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