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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-1-19
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pubmed:abstractText |
To investigate the feasibility of repeated gene transfection in suicide gene therapy against human solid tumors by a combination of 5- fluorocytosine (5-FC) and its converting enzyme, cytosine deaminase (CD), we repeatedly transfected the yeast CD gene into the human pancreatic cancer cell line BXPC3 using the hemagglutinating virus of Japan-liposome in a new gene transfer method. The in vivo growth of the s.c. transplanted BXPC3 tumor in nude mice given CD-gene transfection was significantly suppressed by i.p. injection of 5-FC when compared with tumors treated with the control vector. Furthermore, the tumor transfected with the CD gene during a 7-day interval was suppressed much more than that of a single transfection. These results suggest that repeated transfection of the suicide gene together with the combination of 5-FC and the yeast CD gene using the hemagglutinating virus of Japan-liposome gene transfer method may be useful for the treatment of human solid tumors, including pancreatic cancer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0008-5472
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pubmed:author |
pubmed-author:AiharaTT,
pubmed-author:KanedaYY,
pubmed-author:KanyamaHH,
pubmed-author:MiyoshiYY,
pubmed-author:MondenMM,
pubmed-author:OhueMM,
pubmed-author:SakitaII,
pubmed-author:SekimotoMM,
pubmed-author:SenterP DPD,
pubmed-author:TamakiYY,
pubmed-author:TomitaNN,
pubmed-author:YamanoTT
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11196152-Animals,
pubmed-meshheading:11196152-Antimetabolites, Antineoplastic,
pubmed-meshheading:11196152-Cell Division,
pubmed-meshheading:11196152-Cytosine Deaminase,
pubmed-meshheading:11196152-Feasibility Studies,
pubmed-meshheading:11196152-Female,
pubmed-meshheading:11196152-Flucytosine,
pubmed-meshheading:11196152-Fluorouracil,
pubmed-meshheading:11196152-Gene Therapy,
pubmed-meshheading:11196152-Genetic Vectors,
pubmed-meshheading:11196152-Humans,
pubmed-meshheading:11196152-Injections, Intralesional,
pubmed-meshheading:11196152-Liposomes,
pubmed-meshheading:11196152-Mice,
pubmed-meshheading:11196152-Mice, Inbred BALB C,
pubmed-meshheading:11196152-Mice, Nude,
pubmed-meshheading:11196152-Nucleoside Deaminases,
pubmed-meshheading:11196152-Pancreatic Neoplasms,
pubmed-meshheading:11196152-RNA, Messenger,
pubmed-meshheading:11196152-Respirovirus,
pubmed-meshheading:11196152-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11196152-Transfection,
pubmed-meshheading:11196152-Tumor Cells, Cultured,
pubmed-meshheading:11196152-Xenograft Model Antitumor Assays
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pubmed:year |
2001
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pubmed:articleTitle |
Usefulness of repeated direct intratumoral gene transfer using hemagglutinating virus of Japan-liposome method for cytosine deaminase suicide gene therapy.
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pubmed:affiliation |
Department of Surgery II, Osaka University Medical School, Suita, Japan. h-kan@qf6.s0-net.nc.jp
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pubmed:publicationType |
Journal Article
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