Linked Life Data

11191912

Source:http://linkedlifedata.com/resource/pubmed/id/11191912

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-1-15
pubmed:abstractText
Parasites are often found in a milieu that requires extensive preparation and labor-intensive cleaning before they are suitable for use in analytical procedures. Application of modern techniques in immunology and molecular biology demands pure yields of parasites. To purify first-stage (L1) larvae of Elaphostrongylus cervi, fecal suspensions from an infected red deer were processed by the Baermann method and embedded in a gel matrix with the objective of selectively trapping fecal debris. About half the number (50.9%) of embedded larvae migrated out of the gel within a 24-hr period and were collected as clean parasite suspensions, virtually free from fecal debris. The numbers of L1 emigrating from gels were inversely proportional to the fecal debris content and the thickness of the gel. Removal of fecal debris from Baermann fluid by sieving prior to gel embedment enhanced the yield of pure L1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-3395
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1336-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Purification of first-stage larvae of Elaphostrongylus cervi (Nematoda: Protostrongylidae) from feces.
pubmed:affiliation
Centre for Animal Parasitology, Canadian Food Inspection Agency, Saskatoon, Saskatchewan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't