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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-1-12
pubmed:abstractText
The aim of this study was to characterize the transport of the organic cation 1-methyl-4-phenylpyridinium (MPP+) in an immortalized cell line of rat capillary cerebral endothelial cells (RBE 4). Verapamil (100 microM) and rhodamine 123 (10 microM), and decynium22 (2 microM) and corticosterone (100 microM) reduced cellular accumulation of [3H]MPP+ applied from the luminal and abluminal cell border, respectively. When cells were grown on plastic supports, [3H]MPP+ accumulated in the cells. The kinetic parameters of the saturable component were: Km=25 microM and Vmax=246 pmol per mg protein and 15 min. A selective organic anion transport inhibitor and selective inhibitors of the L- and A-type amino acid transporters did not affect [3H]MPP+ uptake. Uptake of [3H]MPP+ was Na+-independent and metabolic energy-, pH- and potential-dependent. It was inhibited by several organic cations (e.g., verapamil, quinidine, daunomycin, dopamine) but not by others (cimetidine, tetraethylammonium, N-methylnicotinamide). In conclusion, [3H]MPP+ is efficiently transported by RBE 4 cells in both abluminal-to-luminal and luminal-to-abluminal directions. Absorption of [3H]MPP+ seems to occur through a carrier-mediated mechanism belonging to the amphiphilic solute facilitator (ASF) family of transporters, but distinct from the known members of this family.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0028-1298
pubmed:author
pubmed:issnType
Print
pubmed:volume
363
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11191826-1-Methyl-4-phenylpyridinium, pubmed-meshheading:11191826-2,4-Dichlorophenoxyacetic Acid, pubmed-meshheading:11191826-Amiloride, pubmed-meshheading:11191826-Animals, pubmed-meshheading:11191826-Antibodies, Monoclonal, pubmed-meshheading:11191826-Biological Transport, pubmed-meshheading:11191826-Brain, pubmed-meshheading:11191826-Cations, pubmed-meshheading:11191826-Cell Line, pubmed-meshheading:11191826-Dose-Response Relationship, Drug, pubmed-meshheading:11191826-Endothelium, Vascular, pubmed-meshheading:11191826-Energy Metabolism, pubmed-meshheading:11191826-Epithelial Cells, pubmed-meshheading:11191826-Hydrogen-Ion Concentration, pubmed-meshheading:11191826-Kinetics, pubmed-meshheading:11191826-Membrane Potentials, pubmed-meshheading:11191826-Ouabain, pubmed-meshheading:11191826-P-Glycoprotein, pubmed-meshheading:11191826-Rats, pubmed-meshheading:11191826-Sodium, pubmed-meshheading:11191826-Time Factors, pubmed-meshheading:11191826-Tritium
pubmed:year
2001
pubmed:articleTitle
Transport of [3H]MPP+ in an immortalized rat brain microvessel endothelial cell line (RBE 4).
pubmed:affiliation
Department of Biochemistry, Faculty of Medicine, Porto, Portugal. fatima_martel@hotmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't