Linked Life Data

11188759

Source:http://linkedlifedata.com/resource/pubmed/id/11188759

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:11188759rdf:typepubmed:Citationlld:pubmed
pubmed-article:11188759lifeskim:mentionsumls-concept:C0014834lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C0035681lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C0021467lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C1145667lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C1996904lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C0021469lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C1880497lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C1546857lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C0003980lld:lifeskim
pubmed-article:11188759lifeskim:mentionsumls-concept:C0211832lld:lifeskim
pubmed-article:11188759pubmed:issue5lld:pubmed
pubmed-article:11188759pubmed:dateCreated2001-1-8lld:pubmed
pubmed-article:11188759pubmed:abstractTextThe bacteriophage T4 AsiA protein inhibits transcription from host and phage early promoters and is required, along with the T4 MotA protein, for activation of phage middle promoters. During infection, AsiA is found in a tight association with the sigma70 subunit of RNA polymerase. We show that AsiA binds rapidly to free sigma70 at either 4 degrees C or 30 degrees C to form an AsiA-sigma70 complex that with core efficiently reconstitutes the AsiA-inhibited RNA polymerase. In contrast, AsiA does not inhibit transcription after a 15 minute incubation with RNA polymerase holoenzyme at 4 degrees C, and at 30 degrees C an incubation of several minutes is required to inhibit most of the polymerase. We show that the heat step needed for AsiA is not the formation of an active AsiA protein. However, it is consistent with the momentary dissociation of holoenzyme to give free sigma70 and core. Our results indicate that AsiA is either unable to access holoenzyme directly or does so very slowly. Efficient generation of the AsiA-inhibited RNA polymerase requires that AsiA first binds to free sigma70 and then the AsiA-sigma70 complex binds to core to form the Asi-A-inhibited polymerase.lld:pubmed
pubmed-article:11188759pubmed:languageenglld:pubmed
pubmed-article:11188759pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:citationSubsetIMlld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11188759pubmed:statusMEDLINElld:pubmed
pubmed-article:11188759pubmed:monthDeclld:pubmed
pubmed-article:11188759pubmed:issn0022-2836lld:pubmed
pubmed-article:11188759pubmed:authorpubmed-author:HintonD MDMlld:pubmed
pubmed-article:11188759pubmed:authorpubmed-author:VuthooriSSlld:pubmed
pubmed-article:11188759pubmed:issnTypePrintlld:pubmed
pubmed-article:11188759pubmed:day15lld:pubmed
pubmed-article:11188759pubmed:volume304lld:pubmed
pubmed-article:11188759pubmed:ownerNLMlld:pubmed
pubmed-article:11188759pubmed:authorsCompleteYlld:pubmed
pubmed-article:11188759pubmed:pagination731-9lld:pubmed
pubmed-article:11188759pubmed:dateRevised2008-11-21lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:meshHeadingpubmed-meshheading:11188759...lld:pubmed
pubmed-article:11188759pubmed:year2000lld:pubmed
pubmed-article:11188759pubmed:articleTitleEfficient inhibition of Escherichia coli RNA polymerase by the bacteriophage T4 AsiA protein requires that AsiA binds first to free sigma70.lld:pubmed
pubmed-article:11188759pubmed:affiliationLaboratory of Molecular and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA. dhinton@helix.nih.govlld:pubmed
pubmed-article:11188759pubmed:publicationTypeJournal Articlelld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11188759lld:pubmed