Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-2-22
pubmed:abstractText
A native immunoisolation procedure has been used to investigate the role of clathrin-coated vesicles (CCVs) in the transport of vacuolar proteins between the trans-Golgi network (TGN) and the prevacuolar/endosome compartments in the yeast Saccharomyces cerevisiae. We find that Apl2p, one large subunit of the adaptor protein-1 complex, and Vps10p, the carboxypeptidase Y vacuolar protein receptor, are associated with clathrin molecules. Vps10p packaging in CCVs is reduced in pep12 Delta and vps34 Delta, two mutants that block Vps10p transport from the TGN to the endosome. However, Vps10p sorting is independent of Apl2p. Interestingly, a Vps10C(t) Delta p mutant lacking its C-terminal cytoplasmic domain, the portion of the receptor responsible for carboxypeptidase Y sorting, is also coimmunoprecipitated with clathrin. Our results suggest that CCVs mediate Vps10p transport from the TGN to the endosome independent of direct interactions between Vps10p and clathrin coats. The Vps10p C-terminal domain appears to play a principal role in retrieval of Vps10p from the prevacuolar compartment rather than in sorting from the TGN.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-10352010, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-10406795, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-10472183, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-10564262, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-10601336, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-10628971, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-10679018, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-1322413, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-1400571, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-1493334, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-1527174, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-1639056, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-2172253, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-2180925, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-2211819, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-2247081, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-237205, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-2476806, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-2545438, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-2675311, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-2676511, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-3062374, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-3288647, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-3536126, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-3538017, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-7593183, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-7608087, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-7615679, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-7640295, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-7698993, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-7721937, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-7749194, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-7989323, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8157009, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8187177, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8257795, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8385367, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8534908, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8636229, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8730101, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8909536, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8930891, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-8955161, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9015300, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9128246, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9171338, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9184222, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9201718, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9242653, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9242916, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9245784, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9261055, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9314526, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9335339, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9412470, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9548715, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9564031, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9650782, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9714809, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9722605, http://linkedlifedata.com/resource/pubmed/commentcorrection/11179429-9812899
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Protein Complex beta..., http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid Endopeptidases, http://linkedlifedata.com/resource/pubmed/chemical/Clathrin, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/KEX2 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/PEP1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/PEP12 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Proprotein Convertases, http://linkedlifedata.com/resource/pubmed/chemical/Qa-SNARE Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Subtilisins, http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins, http://linkedlifedata.com/resource/pubmed/chemical/aspartic proteinase A
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1059-1524
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
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