Source:http://linkedlifedata.com/resource/pubmed/id/11177599
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
2001-2-22
|
pubmed:abstractText |
The aim of this study was to evaluate the specificity of a number of monoclonal antibodies (MAbs) used for immunological in vitro purging of stem cell grafts from neuroblastoma patients. The extent of cross-reactivity of 10 neuroblastoma-specific MAbs (NB-MAb) with CD34+ stem cells from 14 leukapheresis products was analyzed. The level of cross-reactivity was analyzed on a Coulter (Fullerton, CA) flow cytometer using biotinylated NB-MAbs. There was a marked difference in the reactivity of the ten NB-MAbs with CD34+ stem cells. The antibodies could be divided into three groups with increasing levels of cross reactivity. Four antibodies (126-4, 5.1 H11, UJ127.11, and 14.G2a) all reacted with median levels of less than 2% (range 0.0 to 5.4) of CD34+ stem cells (median of 14 patients). Another three antibodies reacted with a median of 3.1-4.1% of the stem cells (UJ13A, Ab390, and Ab459) but with a wide range (0.2 to 25.6). Finally, M340, HSAN 1.2, and antiThy-1 reacted with a median of 9-16% of the stem cells (range 0.6 to 51.5). We conclude that there is a significant variation in the proportion of CD34+ stem cells reacting with each of the ten neuroblastoma antibodies investigated in this study. Therefore, to avoid a significant loss of CD34+ cells from the stem cell product, we find it important to carefully consider which antibodies to use for immunomagnetic purging.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
1525-8165
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
9
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
867-75
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:11177599-Animals,
pubmed-meshheading:11177599-Antibodies, Monoclonal,
pubmed-meshheading:11177599-Antibody Specificity,
pubmed-meshheading:11177599-Antigens, CD34,
pubmed-meshheading:11177599-Blood Component Removal,
pubmed-meshheading:11177599-Cross Reactions,
pubmed-meshheading:11177599-Flow Cytometry,
pubmed-meshheading:11177599-Hematopoietic Stem Cells,
pubmed-meshheading:11177599-Mice,
pubmed-meshheading:11177599-Neuroblastoma,
pubmed-meshheading:11177599-Tumor Cells, Cultured
|
pubmed:year |
2000
|
pubmed:articleTitle |
Monoclonal antibodies with neuroblastoma specificity: a flow cytometric analysis of cross-reactivity with CD34+ hematopoietic stem cells.
|
pubmed:affiliation |
Clinical Immunological Department, Bloodbank 2034, Laboratory Centre, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark. ifversen@rh.dk
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|