Linked Life Data

11173852

Source:http://linkedlifedata.com/resource/pubmed/id/11173852

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-4
pubmed:dateCreated
2001-2-22
pubmed:databankReference
pubmed:abstractText
We have previously isolated and characterized the mouse Testicular Haploid Expressed Gene (Theg) that is specifically expressed in haploid germ cells. We now describe the molecular cloning and characterization of the human homologue (THEG) of mouse Theg. Expression studies by using both dot blot and Northern blot techniques revealed that human THEG is expressed specifically in the testis. Additionally, we found two alternatively spliced transcripts (THEG major and THEG minor) for THEG by using reverse transcription-polymerase chain reaction on human testicular RNA. Sequence analysis of these PCR products demonstrated that the smaller transcript (THEG minor) lacks 72 bp which was also observed for the mouse Theg. We have isolated the cDNAs of human THEG major and THEG minor, containing the complete open reading frames, which encode putative nuclear proteins of 379 amino acids and 355 amino acids, respectively. Database searches identified two genomic clones on chromosome 19 harboring the human THEG gene, which is approximately 14 kb pairs in size, contains eight exons, and comparison of the two cDNA sequences with the genomic sequence indicated that the smaller transcript lacks exon 3. Furthermore, we assigned the human THEG gene (THEG) to human chromosome 19ptel--> p13 by fluorescence in situ hybridization. Moreover, we detected mouse THEG protein prominently in the nucleus of round spermatids by using an antibody against THEG on both testicular sections and cellular suspensions. Additionally, the subcellular localization of mouse THEG was confirmed by a green fluorescent protein (GFP) fusion protein of mouse THEG which was found mainly in the nucleus of transfected NIH3T3 cells. These data suggest that both human and mouse THEG are specifically expressed in the nucleus of haploid male germ cells and are involved in the regulation of nuclear functions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-0171
pubmed:author
pubmed:copyrightInfo
Copyright 2001 S. Karger AG, Basel
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11173852-Alternative Splicing, pubmed-meshheading:11173852-Amino Acid Sequence, pubmed-meshheading:11173852-Animals, pubmed-meshheading:11173852-Base Sequence, pubmed-meshheading:11173852-Carrier Proteins, pubmed-meshheading:11173852-Chromosomes, Human, Pair 19, pubmed-meshheading:11173852-Cloning, Molecular, pubmed-meshheading:11173852-Exons, pubmed-meshheading:11173852-Expressed Sequence Tags, pubmed-meshheading:11173852-Humans, pubmed-meshheading:11173852-Immunohistochemistry, pubmed-meshheading:11173852-In Situ Hybridization, Fluorescence, pubmed-meshheading:11173852-Introns, pubmed-meshheading:11173852-Mice, pubmed-meshheading:11173852-Molecular Sequence Data, pubmed-meshheading:11173852-Physical Chromosome Mapping, pubmed-meshheading:11173852-Protein Transport, pubmed-meshheading:11173852-RNA, Messenger, pubmed-meshheading:11173852-Recombinant Fusion Proteins
pubmed:year
2000
pubmed:articleTitle
Alternative splicing, chromosome assignment and subcellular localization of the testicular haploid expressed gene (THEG).
pubmed:affiliation
Institute of Human Genetics, University of Göttingen, Göttingen , Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't