Linked Life Data

11172683

Source:http://linkedlifedata.com/resource/pubmed/id/11172683

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-2-22
pubmed:abstractText
Cutaneous wound healing is a complex process involving interactions of various cell types. Skin, in addition to certain other organs, is dependent on estrogen; and estrogen-deficiency is associated with impaired wound healing. Wound healing involves the action of collagenolytic matrix metalloproteinases (MMPs). We investigated the expression and localization of collagenolytic MMPs -8 and -13 by collagenase activity assay, Western immunoblot analysis, in situ hybridization and immunohistochemical staining as well as type I collagen by hydroxyproline content analysis and immunohistochemical staining in cutaneous wounds from aged Sham and ovarioectomized (OVX) rats. After wounding, OVX rats were treated with either placebo, chemically modified tetracycline-8 (CMT-8) or estrogen. We found that MMP-8 and MMP-13 mRNA were expressed in wound epithelium of all samples examined as evidenced by in situ hybridization. Type I collagen, which was abundant in all groups examined, was decreased in OVX rats, but was increased by both CMT-8 and estrogen treatments to the level of Sham group. Hydroxyproline analysis revealed similar results. Western blot data showed that all forms of MMP-8 and MMP-13 were clearly reduced in the CMT-8 treated group compared to OVX. Analysis of collagenolytic activity confirmed the decreased collagenolysis in skin wound extracts from CMT-treated rats when compared with skin wound extracts from OVX rats. Our results show for the first time that MMP-8 mRNA and protein are expressed in rat wound epithelium. We further show that CMT-8 and estrogen have a beneficial effect on skin wound healing in OVX rats by increasing the collagen content and reducing the MMP-mediated collagenolysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0929-8673
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-94
pubmed:dateRevised
2007-2-12
pubmed:meshHeading
pubmed-meshheading:11172683-Animals, pubmed-meshheading:11172683-Collagen, pubmed-meshheading:11172683-Collagenases, pubmed-meshheading:11172683-Epithelial Cells, pubmed-meshheading:11172683-Estrogens, pubmed-meshheading:11172683-Female, pubmed-meshheading:11172683-Gene Expression Regulation, pubmed-meshheading:11172683-In Situ Hybridization, pubmed-meshheading:11172683-Matrix Metalloproteinase 13, pubmed-meshheading:11172683-Matrix Metalloproteinase 8, pubmed-meshheading:11172683-Ovariectomy, pubmed-meshheading:11172683-Protease Inhibitors, pubmed-meshheading:11172683-Rats, pubmed-meshheading:11172683-Rats, Sprague-Dawley, pubmed-meshheading:11172683-Skin, pubmed-meshheading:11172683-Tetracyclines, pubmed-meshheading:11172683-Transcription, Genetic, pubmed-meshheading:11172683-Wound Healing, pubmed-meshheading:11172683-Wounds and Injuries
pubmed:year
2001
pubmed:articleTitle
Wound healing in ovariectomized rats: effects of chemically modified tetracycline (CMT-8) and estrogen on matrix metalloproteinases -8, -13 and type I collagen expression.
pubmed:affiliation
Department of Clinical Veterinary Sciences, University of Helsinki, Helsinki, Finland. pirila@hammas.helsinki.fi
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't