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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1975-6-2
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pubmed:abstractText |
A direct colorimetric assay for total conjugated taurine from bile-rich duodenal aspirates is described. The method is based on complete acetylation of the free hydroxyl groups by acetic anhydride at 100 degrees C. of both the tri- and di-hydroxy bile acids in Folch extracted bile samples. Taurine-conjugated bile acids are measured by ion pair formation with Azure A and subsequent extraction of the complex into the organic phase of a biphasic system. Absorption at 645 nm. of this complex directly quantifies total taurine-conjugated bile acid. Total bile acids are then estimated by the 3alpha-hydroxysteroid dehydrogenase assay. The difference between the concentration of the total conjugated bile acid and of the total conjugated taurine determines the concentration of glycine conjugates and the glycine:taurine ratio. Potentially interfering materials such as sulphalipids, certain phospholipids, and unconjugated bile acids are removed by Folch extraction. The 3-hydroxysteroid sulfates (cholesteryl sulfate, lithocholate sulfate, and glycocholate sulfate) are not measurable by heating in acetic anhydride and do not interfere. Taurolithocholate-3-sulfate, under identical conditions, gives a measurable but very low color yield and in normal physiologic concentrations would contribute negligible color. As previously reported, this assay under prescribed conditions is selective for long-chain amphipathic sulfates or sulfonates with no measurable color yield with glycine conjugates, unconjugated bile acids, free fatty acids, or lecithin. Values for glycine:taurine ratios by the above-described method in both normal bile extracts and extracts from patients with either elevated or depressed ratios relate closely to those determined by thin-layer chromatography.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Anhydrides,
http://linkedlifedata.com/resource/pubmed/chemical/Bile Acids and Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Imines,
http://linkedlifedata.com/resource/pubmed/chemical/Phenothiazines,
http://linkedlifedata.com/resource/pubmed/chemical/Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Taurine,
http://linkedlifedata.com/resource/pubmed/chemical/Taurocholic Acid
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-2143
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
505-14
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1117210-Acetic Acids,
pubmed-meshheading:1117210-Acetylation,
pubmed-meshheading:1117210-Anhydrides,
pubmed-meshheading:1117210-Bile,
pubmed-meshheading:1117210-Bile Acids and Salts,
pubmed-meshheading:1117210-Chromatography, Thin Layer,
pubmed-meshheading:1117210-Color,
pubmed-meshheading:1117210-Colorimetry,
pubmed-meshheading:1117210-Glycine,
pubmed-meshheading:1117210-Humans,
pubmed-meshheading:1117210-Imines,
pubmed-meshheading:1117210-Phenothiazines,
pubmed-meshheading:1117210-Solutions,
pubmed-meshheading:1117210-Taurine,
pubmed-meshheading:1117210-Taurocholic Acid
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pubmed:year |
1975
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pubmed:articleTitle |
Non chromatographic colorimetric assay for total taurine-conjugated bile acids: application of measurements of glycine: taurine ratio in bile.
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pubmed:publicationType |
Journal Article,
Comparative Study
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