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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-2-26
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pubmed:abstractText |
Intracellular water concentrations in single living cells were visualized by nonlinear coherent anti-Stokes Raman scattering (CARS) microscopy. In combination with isotopic exchange measurements, CARS microscopy allowed the real-time observation of transient intracellular hydrodynamics at a high spatial resolution. Studies of the hydrodynamics in the microorganism Dictyostelium discoideum indicated the presence of a microscopic region near the plasma membrane where the mobility of water molecules is severely restricted. Modeling the transient hydrodynamics eventuated in the determination of cell-specific cytosolic diffusion and plasma membrane permeability constants. Our experiments demonstrate that CARS microscopy offers an invaluable tool for probing single-cell water dynamics.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-10080269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-10680682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-2321027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-2540807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-3224144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-5411117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-6252271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-7626740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-8539621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-8722821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-9177195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-9251802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-9305756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-9412473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171993-9608585
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1577-82
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:11171993-Animals,
pubmed-meshheading:11171993-COS Cells,
pubmed-meshheading:11171993-Cell Membrane Permeability,
pubmed-meshheading:11171993-Cercopithecus aethiops,
pubmed-meshheading:11171993-Deuterium Oxide,
pubmed-meshheading:11171993-Dictyostelium,
pubmed-meshheading:11171993-Diffusion,
pubmed-meshheading:11171993-Fluorescent Dyes,
pubmed-meshheading:11171993-Image Processing, Computer-Assisted,
pubmed-meshheading:11171993-Intracellular Fluid,
pubmed-meshheading:11171993-Naphthalenes,
pubmed-meshheading:11171993-Time Factors,
pubmed-meshheading:11171993-Water
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pubmed:year |
2001
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pubmed:articleTitle |
Real-time visualization of intracellular hydrodynamics in single living cells.
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pubmed:affiliation |
Ultrafast Laser and Spectroscopy Laboratory, Materials Science Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
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pubmed:publicationType |
Journal Article
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