Source:http://linkedlifedata.com/resource/pubmed/id/11171616
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2001-2-22
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| pubmed:abstractText |
Membrane Cl(-) channels play an important role in cell volume homeostasis and regulation of volume-sensitive cell transport and metabolism. Heterologous expression of ClC-2 channel cDNA leads to the appearance of swelling-activated Cl(-) currents, consistent with a role in cell volume regulation. Since channel properties in heterologous models are potentially modified by cellular background, we evaluated whether endogenous ClC-2 proteins are functionally important in cell volume regulation. As shown by whole cell patch clamp techniques in rat HTC hepatoma cells, cell volume increases stimulated inwardly rectifying Cl(-) currents when non-ClC-2 currents were blocked by DIDS (100 microM). A cDNA closely homologous with rat brain ClC-2 was isolated from HTC cells; identical sequence was demonstrated for ClC-2 cDNAs in primary rat hepatocytes and cholangiocytes. ClC-2 mRNA and membrane protein expression was demonstrated by in situ hybridization, immunocytochemistry, and Western blot. Intracellular delivery of antibodies to an essential regulatory domain of ClC-2 decreased ClC-2-dependent currents expressed in HEK-293 cells. In HTC cells, the same antibodies prevented activation of endogenous Cl(-) currents by cell volume increases or exposure to the purinergic receptor agonist ATP and delayed HTC cell volume recovery from swelling. These studies provide further evidence that mammalian ClC-2 channel proteins are functional and suggest that in HTC cells they contribute to physiological changes in membrane Cl(-) permeability and cell volume homeostasis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/ClC-2 chloride channels,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0193-1857
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
280
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
G344-53
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11171616-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:11171616-Animals,
pubmed-meshheading:11171616-Antibodies,
pubmed-meshheading:11171616-Carcinoma, Hepatocellular,
pubmed-meshheading:11171616-Cell Line,
pubmed-meshheading:11171616-Cell Membrane,
pubmed-meshheading:11171616-Cell Size,
pubmed-meshheading:11171616-Chloride Channels,
pubmed-meshheading:11171616-Chlorides,
pubmed-meshheading:11171616-DNA, Complementary,
pubmed-meshheading:11171616-Epithelial Cells,
pubmed-meshheading:11171616-Hepatocytes,
pubmed-meshheading:11171616-Homeostasis,
pubmed-meshheading:11171616-Humans,
pubmed-meshheading:11171616-Microinjections,
pubmed-meshheading:11171616-Patch-Clamp Techniques,
pubmed-meshheading:11171616-Rats,
pubmed-meshheading:11171616-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11171616-Transfection
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| pubmed:year |
2001
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| pubmed:articleTitle |
ClC-2 chloride channels contribute to HTC cell volume homeostasis.
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| pubmed:affiliation |
Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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