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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 3
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pubmed:dateCreated |
2001-2-22
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pubmed:abstractText |
Two mono- and a di-RNA-cleaving DNA enzymes with the 10-23 catalytic motif were synthesized that were targeted to cleave at the conserved site/sites of the X gene of the hepatitis B virus. In each case, protein-independent but Mg(2+)-dependent cleavage of in vitro-synthesized full-length X RNA was obtained. Specific cleavage products were obtained with two different mono- and a di-DNA enzyme, with the latter giving rise to multiple RNA fragments that retained the cleavage specificity of the mono-DNA enzymes. A relatively less efficient cleavage was also obtained under simulated physiological conditions by the two mono-DNA enzymes but the efficiency of the di-DNA enzyme was significantly reduced. A single nucleotide change (G to C) in the 10-23 catalytic motif of the DNA enzyme 307 abolished its ability to cleave target RNA completely. Both, mono- and di-DNA enzymes, when introduced into a mammalian cell, showed specific inhibition of X-gene-mediated transactivation of reporter-gene expression. This decrease was due to the ability of these DNA enzymes to cleave X RNA intracellularly, which was also reflected by significant reduction in the levels of X protein in a liver-specific cell line, HepG2. Ribonuclease protection assay confirmed the specific reduction of X RNA in DNA-enzyme-treated cells. Potential in vivo applications of mono- and di-DNA enzymes in interfering specifically with the X-gene-mediated pathology are discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-1408760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-1529527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-1583465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-1738200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-2107573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-2219734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-2438771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-2441261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-2457170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-2538828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-3016298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-3201754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-7479038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-7544984,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-7648028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-8188703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-8570629,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-9113977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-9114724,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11171068-9781685
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
353
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
701-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11171068-Animals,
pubmed-meshheading:11171068-Base Sequence,
pubmed-meshheading:11171068-COS Cells,
pubmed-meshheading:11171068-Cell Line,
pubmed-meshheading:11171068-DNA Primers,
pubmed-meshheading:11171068-Deoxyribonucleases,
pubmed-meshheading:11171068-Down-Regulation,
pubmed-meshheading:11171068-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11171068-HIV Long Terminal Repeat,
pubmed-meshheading:11171068-Humans,
pubmed-meshheading:11171068-Kinetics,
pubmed-meshheading:11171068-Promoter Regions, Genetic,
pubmed-meshheading:11171068-Trans-Activators,
pubmed-meshheading:11171068-Transcriptional Activation
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pubmed:year |
2001
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pubmed:articleTitle |
Inhibition of hepatitis B virus X gene expression by novel DNA enzymes.
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pubmed:affiliation |
Laboratory of Virology, National Institute of Immunology, JNU Campus, Aruna Asaf Ali Marg, New Delhi-110067, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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