Source:http://linkedlifedata.com/resource/pubmed/id/11170976
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2001-2-22
|
| pubmed:abstractText |
Volunteers experimentally infected with influenza A/Texas/36/91 (H1N1) virus and treated with the neuraminidase (NA) inhibitor oseltamivir were monitored for the emergence of drug-resistant variants. Two (4%) of 54 resistant viruses were detected by NA inhibition assay among last-day isolates recovered from 54 drug recipients. They bore a substitution His274Tyr in the NA. Hemagglutinin (HA) variants detected in the placebo group differed from the egg-adapted inoculum virus by virtue of amino acid substitutions at residues 137, 225, or both. These variants had a higher affinity for Neu5Ac(alpha2-6)Gal-containing receptors, which are characteristic of human respiratory epithelium, than for Neu5Ac(alpha2-3)Gal-containing receptors, which are typical of chicken egg allantoic membrane. Although appearing to be more sensitive to oseltamivir in humans, the variants with increased affinity for Neu5Ac(alpha2-6)Gal receptors were less sensitive than the Neu5Ac(alpha2-3)Gal-binding variants in Madin-Darby canine kidney cells. Thus, HA affinity for receptors is an essential feature of influenza virus susceptibility to NA inhibitors, both in cell culture and in humans.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetamides,
http://linkedlifedata.com/resource/pubmed/chemical/Antiviral Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Hemagglutinin Glycoproteins...,
http://linkedlifedata.com/resource/pubmed/chemical/Neuraminidase,
http://linkedlifedata.com/resource/pubmed/chemical/Oseltamivir,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Virus
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-1899
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
183
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
523-31
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11170976-Acetamides,
pubmed-meshheading:11170976-Adolescent,
pubmed-meshheading:11170976-Adult,
pubmed-meshheading:11170976-Amino Acid Substitution,
pubmed-meshheading:11170976-Animals,
pubmed-meshheading:11170976-Antiviral Agents,
pubmed-meshheading:11170976-Base Sequence,
pubmed-meshheading:11170976-Cell Line,
pubmed-meshheading:11170976-Dogs,
pubmed-meshheading:11170976-Drug Resistance, Microbial,
pubmed-meshheading:11170976-Genetic Variation,
pubmed-meshheading:11170976-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:11170976-Humans,
pubmed-meshheading:11170976-Influenza, Human,
pubmed-meshheading:11170976-Influenza A virus,
pubmed-meshheading:11170976-Microbial Sensitivity Tests,
pubmed-meshheading:11170976-Molecular Sequence Data,
pubmed-meshheading:11170976-Mutation,
pubmed-meshheading:11170976-Neuraminidase,
pubmed-meshheading:11170976-Oseltamivir,
pubmed-meshheading:11170976-Receptors, Virus,
pubmed-meshheading:11170976-Viral Plaque Assay
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Selection of influenza virus mutants in experimentally infected volunteers treated with oseltamivir.
|
| pubmed:affiliation |
Department of Internal Medicine, Division of Epidemiology and Virology, University of Virginia, PO Box 473, Charlottesville, VA 22908, USA. lvg9b@virginia.edu
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| pubmed:publicationType |
Journal Article,
Clinical Trial,
Research Support, U.S. Gov't, P.H.S.,
Randomized Controlled Trial,
Research Support, Non-U.S. Gov't
|