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pubmed:abstractText |
The procedure for operation of a constant-temperature, single-column automated amino acid analyser in the sub-nanomole range is described. The cycle time for a complete analysis is 90 min including equilibration for next cycle. Eluting buffers can be made in the laboratory or commercially available concentrates (Pico-Buffers) can be used. A novel reducing agent, ascorbic acid, incorporated into the column buffers was used to reduce air-stable ninhydrin.
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