Linked Life Data

11169467

Source:http://linkedlifedata.com/resource/pubmed/id/11169467

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-2-22
pubmed:abstractText
A new model of surgical injury for the induction and development of stenosis in common rat carotids is described. This model differs from balloon angioplasty or vein graft systems currently applied on animals to develop stenosis, since it involves the entire vessel wall layers and mimics the injury occurring during arterial grafts, endarterectomy or organ transplantation. At different times following arterial damage, the pattern of expression of genes already known to be involved in the proliferation, differentiation, and apoptosis of smooth muscle cells (c-myc, Angiotensin II receptor 1, Bcl-2 and Bax alpha), as well as of Rb and Rb2 genes, whose pattern of expression after arterial injury has not yet been reported, was analyzed by semi-quantitative reverse transcription-polymerase chain reaction technique. Histological and histochemical analysis on carotid sections shows the morphological changes which occurred 30 days after surgical injury in the vessel wall. Molecular and histological data demonstrate that this model of surgical injury induces neointimal proliferation in about 30% of rats. In about 70% of the remaining rats, it induces the processes responsible for negative remodelling, namely the significant accumulation of extracellular matrix and fibers and disorganization of arterial tunics. This model is therefore available for further studies on the expression of genes involved in the arterial stenotic process, as well as for testing drugs aimed at limiting this recurrent pathophysiological phenomenon.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9541
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
186
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
307-13
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11169467-Animals, pubmed-meshheading:11169467-Apoptosis, pubmed-meshheading:11169467-Carotid Arteries, pubmed-meshheading:11169467-Carotid Stenosis, pubmed-meshheading:11169467-Disease Models, Animal, pubmed-meshheading:11169467-Gene Expression Regulation, pubmed-meshheading:11169467-Genes, Retinoblastoma, pubmed-meshheading:11169467-Genes, bcl-2, pubmed-meshheading:11169467-Genes, myc, pubmed-meshheading:11169467-Muscle, Smooth, Vascular, pubmed-meshheading:11169467-Proto-Oncogene Proteins, pubmed-meshheading:11169467-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:11169467-Rats, pubmed-meshheading:11169467-Rats, Inbred WKY, pubmed-meshheading:11169467-Receptor, Angiotensin, Type 1, pubmed-meshheading:11169467-Receptor, Angiotensin, Type 2, pubmed-meshheading:11169467-Receptors, Angiotensin, pubmed-meshheading:11169467-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11169467-bcl-2-Associated X Protein
pubmed:year
2001
pubmed:articleTitle
Molecular analysis of arterial stenosis in rat carotids.
pubmed:affiliation
Institute of Pharmacology and Toxicology, Second University of Naples, Via Constantinopoli, 16, 80138 Naples, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't