Source:http://linkedlifedata.com/resource/pubmed/id/11169132
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-2-22
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| pubmed:abstractText |
This study aimed at investigating the oral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in head- and neck-irradiated, dentate, xerostomic individuals. Subjects were recruited from a nasopharyngeal carcinoma clinic and were segregated into group A: <60 years (n=25, 48+/-6 years, 5+/-5 years post-irradiation) and group B: >or=60 years (n=8, 67+/-4 years, 2+/-2 years post-irradiation) and were compared with age- and sex-matched normal individuals, group C: <60 years (n=20, 44+/-12 years) and group D: >or=60 years (n=10, 70+/-3 years). Selective culture of the oral rinse samples was carried out to isolate, quantify and speciate (using API 20E kit) aerobic and facultatively anaerobic gram-negative rods and cocci recovery. All test subjects were put under comprehensive oral and preventive care for 3 months, and 12 group A and 5 group B subjects were recalled for reassessment of aerobic and facultatively anaerobic gram-negative rods and cocci colonization. All identical isolates, pre- and post-hygienic care, were phenotypically (Vitek, Hazelwood, MA and antibiogram profile) and genotypically (pulsed-field gel electrophoresis) evaluated. The aerobic and facultatively anaerobic gram-negative rods and cocci isolated from the first round oral rinse samples included: Acinetobacter, Neisseria, Chryseomonas, Flavimonas, Pseudomonas, Citrobacter, Enterobacter, Escherichia, Klebsiella, Flavobacterium and Weeksella species. The aerobic and facultatively anaerobic gram-negative rods and cocci isolation rate was high for irradiated individuals, and they were 64/25% and 100/80% for groups A/C and B/D, respectively. Recovery of aerobic and facultatively anaerobic gram-negative rods and cocci and Klebsiella pneumoniae subsp. pneumoniae in oral rinse samples were found to be significantly more prevalent in the irradiated subjects (groups A and B). Enterobacteriaceae were more frequently isolated from oral rinse samples of aged irradiated subjects (group B vs D, P<0.05), where the quantity of Citrobacter freundii (colony-forming units/ml oral rinse) was also significantly elevated. The isolation rate of aerobic and facultatively anaerobic gram-negative rods and cocci after hygienic care remained unchanged; 3 of 12 and 3 of 5 of the recalled subjects from groups A and B, respectively, harbored same aerobic and facultatively anaerobic gram-negative rods and cocci species. However, only two pairs of K. pneumoniae subsp. pneumoniae, sequentially isolated from same patients in group B, were found to be identical by pulsed-field gel electrophoresis. This may be due to reinfection of the microbes from the same source or permanent colonization. In conclusion, irradiation-induced xerostomia seems to favor frequent, repeated, transient intraoral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
D
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0902-0055
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
16
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1-9
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11169132-Acinetobacter,
pubmed-meshheading:11169132-Adult,
pubmed-meshheading:11169132-Age Factors,
pubmed-meshheading:11169132-Aged,
pubmed-meshheading:11169132-Bacterial Typing Techniques,
pubmed-meshheading:11169132-Carcinoma,
pubmed-meshheading:11169132-Case-Control Studies,
pubmed-meshheading:11169132-Citrobacter,
pubmed-meshheading:11169132-Cohort Studies,
pubmed-meshheading:11169132-DNA, Bacterial,
pubmed-meshheading:11169132-Drug Resistance, Bacterial,
pubmed-meshheading:11169132-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:11169132-Enterobacter,
pubmed-meshheading:11169132-Escherichia,
pubmed-meshheading:11169132-Flavobacterium,
pubmed-meshheading:11169132-Follow-Up Studies,
pubmed-meshheading:11169132-Genotype,
pubmed-meshheading:11169132-Gram-Negative Aerobic Rods and Cocci,
pubmed-meshheading:11169132-Gram-Negative Facultatively Anaerobic Rods,
pubmed-meshheading:11169132-Humans,
pubmed-meshheading:11169132-Klebsiella,
pubmed-meshheading:11169132-Klebsiella pneumoniae,
pubmed-meshheading:11169132-Middle Aged,
pubmed-meshheading:11169132-Mouth,
pubmed-meshheading:11169132-Nasopharyngeal Neoplasms,
pubmed-meshheading:11169132-Neisseria,
pubmed-meshheading:11169132-Oral Hygiene,
pubmed-meshheading:11169132-Phenotype,
pubmed-meshheading:11169132-Pseudomonas,
pubmed-meshheading:11169132-Statistics, Nonparametric,
pubmed-meshheading:11169132-Statistics as Topic,
pubmed-meshheading:11169132-Xerostomia
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| pubmed:year |
2001
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| pubmed:articleTitle |
Oral colonization of aerobic and facultatively anaerobic gram-negative rods and cocci in irradiated, dentate, xerostomic individuals.
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| pubmed:affiliation |
Faculty of Dentistry, Faculty of Medicine, University of Hong Kong, Hong Kong SAR, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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