Source:http://linkedlifedata.com/resource/pubmed/id/11167753
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2001-2-22
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| pubmed:abstractText |
The type of regimen used might result in mobilization of phenotypically and functionally different CD34(+) cells. We compared the phenotype of CD34(+) cells in leukapheresis products of three homogeneous groups: I, healthy individuals treated with granulocyte colony-stimulating factor (G-CSF) alone (n = 13); II, patients mobilized with G-CSF following chemotherapy (n = 16); and III, patients mobilized with G-CSF after high-dose chemotherapeutic pretreatment (n = 24). Multiparameter flow cytometry was performed for CD34(+) subpopulation analysis and focused on adhesion molecules, differentiation markers and megakaryocytic markers relevant for stem cell homing, with special reference to the importance of L-selectin expression. Regimens I and II led to higher numbers of mobilized CD34(+) cells (mean 468 x 10(6) and 491 x 10(6) CD34(+) cells per leukapheresis procedure respectively) than regimen III (mean 41 x 10(6) CD34(+) cells per leukapheresis procedure). Both the expression of L-selectin and CD54 on CD34(+) cells was significantly lower in group III, as was the percentage of megakaryocytic (CD41(+)) progenitors. A higher percentage of primitive (CD38(-) and/or HLA(-)DR(-)) CD34(+) cells was found in group III, correlating with a higher clonogenicity of the CD34(+) cells. However, when comparing the CD34(+)_ subpopulations that were also positive for L-selectin, there was no significant difference between the three regimens. A similar approach for the megakaryocytic CD34+ population resulted in an even worse quality of regimen III: 5.1% of CD34(+) being CD41(+)/L-selectin(+) compared with 9.2% and 8.9% in regimens I and II respectively. We concluded that the phenotypes of the CD34(+) cells in the G-CSF (group I) and G-CSF-chemotherapy (group II) regimens are similar, whereas the phenotype of the CD34(+) cells mobilized in the high-dose regimen (group III) displayed features that might negatively influence homing of the cells. Future studies will be directed towards regimens that will lead to the mobilization of a higher amount of CD34(+) cells with a phenotypically favourable phenotype.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclophosphamide,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Filgrastim,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorouracil,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating...,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Melphalan,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Glycoprotein GPIIb-IIIa...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vincristine
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
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| pubmed:issn |
0007-1048
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
111
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1138-44
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11167753-Antigens, CD34,
pubmed-meshheading:11167753-Antineoplastic Combined Chemotherapy Protocols,
pubmed-meshheading:11167753-Breast Neoplasms,
pubmed-meshheading:11167753-Cyclophosphamide,
pubmed-meshheading:11167753-Dexamethasone,
pubmed-meshheading:11167753-Doxorubicin,
pubmed-meshheading:11167753-Drug Administration Schedule,
pubmed-meshheading:11167753-Flow Cytometry,
pubmed-meshheading:11167753-Fluorouracil,
pubmed-meshheading:11167753-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:11167753-Hematopoietic Stem Cell Mobilization,
pubmed-meshheading:11167753-Humans,
pubmed-meshheading:11167753-Immunophenotyping,
pubmed-meshheading:11167753-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11167753-L-Selectin,
pubmed-meshheading:11167753-Leukapheresis,
pubmed-meshheading:11167753-Megakaryocytes,
pubmed-meshheading:11167753-Melphalan,
pubmed-meshheading:11167753-Multiple Myeloma,
pubmed-meshheading:11167753-Platelet Glycoprotein GPIIb-IIIa Complex,
pubmed-meshheading:11167753-Recombinant Proteins,
pubmed-meshheading:11167753-Stem Cells,
pubmed-meshheading:11167753-Vincristine
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| pubmed:year |
2000
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| pubmed:articleTitle |
The phenotypic profile of CD34-positive peripheral blood stem cells in different mobilization regimens.
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| pubmed:affiliation |
Department of Medical Oncology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands.
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| pubmed:publicationType |
Journal Article
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