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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13-14
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pubmed:dateCreated |
2001-2-22
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pubmed:abstractText |
The early responses of CD4+ T cells to particle-mediated DNA immunisation were investigated using OVA-specific TCR-transgenic CD4+ T cells. Following adoptive transfer of these cells, mice were immunised by delivery into the skin of a plasmid encoding ovalbumin. Transgenic T cells underwent a rapid and transient antigen-specific activation, followed by clonal expansion (up to approximately 6% of total lymphocytes). Immunisation with ovalbumin in CFA evoked similar responses with slightly faster kinetics. Numerous antigen-specific T cells synthesising IFN-gamma (Th1) and IL-4 (Th2) were detectable using both intracellular staining and ELISPOT assays. This study provides a quantitative analysis of both T cell proliferation and Th1/Th2 balance following particle-mediated DNA immunisation and establishes a robust and sensitive model in which to assess modulation of helper T cell responses in DNA vaccination.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1678-87
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11166891-Adoptive Transfer,
pubmed-meshheading:11166891-Animals,
pubmed-meshheading:11166891-Biological Markers,
pubmed-meshheading:11166891-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11166891-Cell Division,
pubmed-meshheading:11166891-Clone Cells,
pubmed-meshheading:11166891-Interferon-gamma,
pubmed-meshheading:11166891-Interleukin-2,
pubmed-meshheading:11166891-Interleukin-4,
pubmed-meshheading:11166891-Interleukin-5,
pubmed-meshheading:11166891-Kinetics,
pubmed-meshheading:11166891-Lymph Nodes,
pubmed-meshheading:11166891-Lymphocyte Activation,
pubmed-meshheading:11166891-Mice,
pubmed-meshheading:11166891-Mice, Inbred BALB C,
pubmed-meshheading:11166891-Mice, Transgenic,
pubmed-meshheading:11166891-Microspheres,
pubmed-meshheading:11166891-Ovalbumin,
pubmed-meshheading:11166891-Receptors, Interleukin-2,
pubmed-meshheading:11166891-Th1 Cells,
pubmed-meshheading:11166891-Th2 Cells,
pubmed-meshheading:11166891-Vaccination,
pubmed-meshheading:11166891-Vaccines, DNA
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pubmed:year |
2001
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pubmed:articleTitle |
Early commitment of adoptively transferred CD4+ T cells following particle-mediated DNA vaccination: implications for the study of immunomodulation.
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pubmed:affiliation |
Department of Immunology, Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, W1P 6DB, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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