Source:http://linkedlifedata.com/resource/pubmed/id/11166031
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2001-2-22
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pubmed:abstractText |
The plasmacytoma cell line, TEPC 2372, was derived from a malignant plasma cell tumor that developed in the peritoneal cavity of a BALB/c mouse that harbored the transgenic shuttle vector for the assessment of mutagenesis in vivo, lambdaLIZ. TEPC 2372 was found to display the typical features of a BALB/c plasmacytoma. It consisted of pleomorphic plasma cells that secreted a monoclonal immunoglobulin (IgG2b/lambda), was initially dependent on the presence of IL-6 to grow in cell culture, contained a hyperdiploid chromosome complement with a tendency to undergo tetraploidization, and harbored a constitutively active c-myc gene by virtue of a T(6;15) chromosomal translocation. TEPC 2372 was further characterized by the ability to respond to in vitro exposure with 4-NQO (4-nitroquinoline-1-oxide), an oxidative model mutagen, with a vigorous dose-dependent increase in mutagenesis that peaked at a 7.85-fold elevation of mutant rates in lambdaLIZ when compared to background mutant rates in untreated controls. Cotreatment with 4-NQO and BSO (buthionine sulfoximine), a glutathione-depleting compound that causes endogenous oxidative stress, resulted in a 9.03-fold increase in the mutant frequency in lambdaLIZ. These results demonstrated that TEPC 2372, the malignant plasma cell counterpart of the lambdaLIZ-based in vivo mutagenesis assay, may be useful as an in vitro reference point for the further elucidation of oxidative mutagenesis in lymphoid tissues.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-5107
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
473
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
121-36
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11166031-Animals,
pubmed-meshheading:11166031-Carcinogens,
pubmed-meshheading:11166031-Cytogenetic Analysis,
pubmed-meshheading:11166031-Genes, myc,
pubmed-meshheading:11166031-Genetic Vectors,
pubmed-meshheading:11166031-Mice,
pubmed-meshheading:11166031-Mice, Inbred C57BL,
pubmed-meshheading:11166031-Mice, Transgenic,
pubmed-meshheading:11166031-Mutagenesis,
pubmed-meshheading:11166031-Mutagenicity Tests,
pubmed-meshheading:11166031-Plasmacytoma,
pubmed-meshheading:11166031-RNA, Messenger,
pubmed-meshheading:11166031-Terpenes,
pubmed-meshheading:11166031-Translocation, Genetic,
pubmed-meshheading:11166031-Tumor Cells, Cultured
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pubmed:year |
2001
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pubmed:articleTitle |
Inducible mutagenesis in TEPC 2372, a mouse plasmacytoma cell line that harbors the transgenic shuttle vector lambdaLIZ.
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pubmed:affiliation |
Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA. felixk@dc37a.nci.nih.gov
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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