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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
2001-2-22
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pubmed:abstractText |
CD98 is a multifunctional heterodimeric membrane protein involved in the regulation of cell adhesion as well as amino acid transport. We show that CD98 cross-linking persistently activates Rap1 GTPase in a LFA-1-dependent manner and induces LFA-1/ICAM-1-mediated cell adhesion in lymphocytes. Specific phosphatidylinositol-3-kinase (PI3K) inhibitors suppressed both LFA-1 activation and Rap1GTP generation, and abrogation of Rap1GTP by retroviral over-expression of a specific Rap1 GTPase activating protein, SPA-1, totally inhibited the LFA-1/ICAM-1-mediated cell adhesion. These results suggest that CD98 cross-linking activates LFA-1 via the PI3K signaling pathway and induces accumulation of Rap1GTP in a LFA-1-dependent manner, which in turn mediates the cytoskeleton-dependent cell adhesion process.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD98,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/rap1 GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
489
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
249-53
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11165259-Androstadienes,
pubmed-meshheading:11165259-Animals,
pubmed-meshheading:11165259-Antibodies, Monoclonal,
pubmed-meshheading:11165259-Antigens, CD,
pubmed-meshheading:11165259-Antigens, CD98,
pubmed-meshheading:11165259-Carrier Proteins,
pubmed-meshheading:11165259-Cell Adhesion,
pubmed-meshheading:11165259-Cell Aggregation,
pubmed-meshheading:11165259-Cell Line,
pubmed-meshheading:11165259-Cells, Cultured,
pubmed-meshheading:11165259-Enzyme Activation,
pubmed-meshheading:11165259-Enzyme Inhibitors,
pubmed-meshheading:11165259-Humans,
pubmed-meshheading:11165259-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11165259-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:11165259-Lymphocytes,
pubmed-meshheading:11165259-Mice,
pubmed-meshheading:11165259-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11165259-rap1 GTP-Binding Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
CD98 induces LFA-1-mediated cell adhesion in lymphoid cells via activation of Rap1.
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pubmed:affiliation |
Department of Immunology and Cell Biology, Graduate School of Medicine, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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