Source:http://linkedlifedata.com/resource/pubmed/id/11164103
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2001-2-22
|
| pubmed:abstractText |
A sufficiently high dose of thrombopoietin to overcome initial c-mpl-mediated clearance stimulates hematopoietic reconstitution following myelosuppressive treatment. We studied the efficacy of thrombopoietin on survival after supralethal total body irradiation (9 Gy) of C57BL6/J mice and the occurrence of infectious and thrombotic complications in comparison with a bone marrow graft or prophylactic antibiotic treatment. Administration of 0.3 microg thrombopoietin, 2 hours after irradiation, protected 62% of the mice as opposed to no survival in placebo controls. A graft with a supraoptimal number of syngeneic bone marrow cells (10(6) cells) fully prevented mortality, whereas antibiotic treatment was ineffective. Blood cell recovery was observed in the thrombopoietin-treated mice but not in the placebo or antibiotic-treated group. Bone marrow and spleen cellularity as well as colony-forming unit granulocyte-macrophage and burst-forming unit erythroid were considerably increased in thrombopoietin-treated mice relative to controls. Histologic examination at day 11 revealed numerous petechiae and vascular obstructions within the brain microvasculature of placebo-treated mice, which was correlated with hypercoagulation and hypofibrinolysis. Thrombopoietin treatment prevented coagulation/fibrinolysis disorder and vascular thrombosis. High fibrinogen levels were related to bacterial infections in 67% of placebo-treated mice and predicted mortality, whereas the majority of the thrombopoietin-treated mice did not show high fibrinogen levels and endotoxin was not detectable in plasma. We conclude that thrombopoietin administration prevents mortality in mice subjected to 9-Gy total body irradiation both by interfering in the cascade leading to thrombotic complications and by amelioration of neutrophil and platelet recovery and thus protects against infections and hemorrhages.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Fibrinogen,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombopoietin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0301-472X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
30-40
|
| pubmed:dateRevised |
2009-11-3
|
| pubmed:meshHeading |
pubmed-meshheading:11164103-Animals,
pubmed-meshheading:11164103-Bacterial Infections,
pubmed-meshheading:11164103-Biological Markers,
pubmed-meshheading:11164103-Blood Coagulation Disorders,
pubmed-meshheading:11164103-Bone Marrow,
pubmed-meshheading:11164103-Bone Marrow Diseases,
pubmed-meshheading:11164103-Disease Susceptibility,
pubmed-meshheading:11164103-Drug Evaluation, Preclinical,
pubmed-meshheading:11164103-Endotoxemia,
pubmed-meshheading:11164103-Fibrinogen,
pubmed-meshheading:11164103-Fibrinolysis,
pubmed-meshheading:11164103-Hemorrhage,
pubmed-meshheading:11164103-Immunologic Deficiency Syndromes,
pubmed-meshheading:11164103-Leukocyte Count,
pubmed-meshheading:11164103-Male,
pubmed-meshheading:11164103-Mice,
pubmed-meshheading:11164103-Mice, Inbred C57BL,
pubmed-meshheading:11164103-Neutrophils,
pubmed-meshheading:11164103-Platelet Activation,
pubmed-meshheading:11164103-Platelet Count,
pubmed-meshheading:11164103-RNA, Messenger,
pubmed-meshheading:11164103-Radiation Injuries, Experimental,
pubmed-meshheading:11164103-Recombinant Proteins,
pubmed-meshheading:11164103-Thrombopoietin,
pubmed-meshheading:11164103-Thrombosis,
pubmed-meshheading:11164103-Whole-Body Irradiation
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Single administration of thrombopoietin to lethally irradiated mice prevents infectious and thrombotic events leading to mortality.
|
| pubmed:affiliation |
Institut de Protection et de Sûreté Nucléaire, IPSN, Fontenay-aux-Roses Cedex, France. marc-andre.mouthon@ipsn.fr
|
| pubmed:publicationType |
Journal Article
|