Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-1-12
pubmed:abstractText
We reported previously that in African green monkey (AGM) CD4 lymphocytes, CD4 mRNA expression undergoes a decrease following in vitro activation, and CD4 cells are therefore subject to loss of CD4 expression on the cell surface. To examine the transcriptional regulation of the CD4 gene in this species. we analyzed the CD4 silencer, which has been identified as a regulatory element responsible for the down regulation of CD4 transcription in CD8 cell lineage cells. Sequence analysis indicated that the CD4 silencer of the AGM was highly homologous to that of humans. However, two nucleotide substitutions were present in one of the nuclear protein binding sites, which was characterized as the FP II site having a strong enhancing effect on transgene expression in CD4 cells. By performing transient transfection assays. we found that the enhancing activities of the CD4 silencer or FP II-containing fragment of the AGM were greatly reduced in a human CD4 cell line as compared to those of human materials. The CD4 mRNA level was significantly decreased in the human CD4 cell line when synthetic oligonucleotide corresponding to the human FP II sequence was added to the culture. These observations imply that FP II-protein interaction might be required for the maintenance of sufficient expression of the CD4 gene, and the enhancing activity mediated by the above interaction might be decreased in the AGM CD4 silencer, due probably to the nucleotide changes occurring at the FP II site.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0165-2478
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-53
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11163866-Animals, pubmed-meshheading:11163866-Antigens, CD4, pubmed-meshheading:11163866-Base Sequence, pubmed-meshheading:11163866-Cell Line, pubmed-meshheading:11163866-Cercopithecus aethiops, pubmed-meshheading:11163866-Down-Regulation, pubmed-meshheading:11163866-Enhancer Elements, Genetic, pubmed-meshheading:11163866-Gene Silencing, pubmed-meshheading:11163866-Genes, MHC Class I, pubmed-meshheading:11163866-Humans, pubmed-meshheading:11163866-Leukocytes, Mononuclear, pubmed-meshheading:11163866-Molecular Sequence Data, pubmed-meshheading:11163866-Oligonucleotides, pubmed-meshheading:11163866-Repressor Proteins, pubmed-meshheading:11163866-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11163866-Sequence Analysis, DNA, pubmed-meshheading:11163866-Silencer Elements, Transcriptional, pubmed-meshheading:11163866-T-Lymphocytes, Helper-Inducer, pubmed-meshheading:11163866-Thymus Gland, pubmed-meshheading:11163866-Transcription, Genetic
pubmed:year
2000
pubmed:articleTitle
Sequence and functional properties of African green monkey CD4 silencer.
pubmed:affiliation
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
pubmed:publicationType
Journal Article