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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2001-2-22
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pubmed:abstractText |
A high-yield production of a peptide vaccine in transgenic plants is described here. A 21-mer peptide, which confers protection to dogs against challenge with virulent canine parvovirus, has been expressed in transgenic plants as an amino-terminal translational fusion with the GUS gene. Transformants were selected on the basis of their GUS activities, showing expression levels of the recombinant protein up to 3% of the total leaf soluble protein, a production yield comparable to that obtained with the same epitope expressed by chimeric plant viruses. The immunogenicity of the plant-derived peptide was demonstrated in mice immunized either intraperitoneally or orally with transgenic plant extracts, providing the suitability of the GUS fusions approach for low-cost production of peptide vaccines.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
488
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11163787-Administration, Oral,
pubmed-meshheading:11163787-Amino Acid Sequence,
pubmed-meshheading:11163787-Animals,
pubmed-meshheading:11163787-Antibodies, Viral,
pubmed-meshheading:11163787-Base Sequence,
pubmed-meshheading:11163787-Dogs,
pubmed-meshheading:11163787-Epitopes,
pubmed-meshheading:11163787-Genes, Reporter,
pubmed-meshheading:11163787-Genetic Engineering,
pubmed-meshheading:11163787-Genetic Vectors,
pubmed-meshheading:11163787-Injections, Intraperitoneal,
pubmed-meshheading:11163787-Mice,
pubmed-meshheading:11163787-Mice, Inbred BALB C,
pubmed-meshheading:11163787-Molecular Sequence Data,
pubmed-meshheading:11163787-Parvovirus,
pubmed-meshheading:11163787-Peptides,
pubmed-meshheading:11163787-Plants,
pubmed-meshheading:11163787-Plants, Genetically Modified,
pubmed-meshheading:11163787-Recombinant Fusion Proteins,
pubmed-meshheading:11163787-Transformation, Genetic,
pubmed-meshheading:11163787-Vaccines, Subunit,
pubmed-meshheading:11163787-Viral Vaccines
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pubmed:year |
2001
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pubmed:articleTitle |
High-yield expression of a viral peptide vaccine in transgenic plants.
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pubmed:affiliation |
Departamento de Mejora Genética y Biotecnología, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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