Linked Life Data

11161617

Source:http://linkedlifedata.com/resource/pubmed/id/11161617

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-2-22
pubmed:abstractText
Galanin is a peptide normally expressed at low levels both in sensory and in sympathetic neurons. It is strongly upregulated after peripheral nerve lesions, and it has been proposed that nerve growth factor (NGF) plays a role in this regulation. In the present study the effect of both sciatic nerve transection and lumbar sympathectomy on galanin in lumbar dorsal root ganglia (DRGs) was examined in mice overexpressing NGF (NGFOE) in the skin under the keratin promoter. The superior cervical ganglia (SCG) were also studied. In the DRG pericellular baskets containing tyrosine hydroxylase- (TH) and galanin-like immunoreactivity (LI) were found, mostly in the same fibers. Galanin-positive baskets were also found in the trigeminal ganglia. However, only single neuropeptide Y (NPY)-positive baskets were observed within the DRGs. No marked difference in number of galanin-positive neurons was seen between wild-type and NGFOE mice. After sciatic nerve transection galanin was upregulated in DRG neurons to about the same extent in NGFOE mice as in wild-type mice. Galanin-, but not TH-LIs decreased in the pericellular baskets. After lumbar sympathectomy both galanin- and TH-immunoreactive baskets disappeared, suggesting a sympathetic origin. In the SCG the very low galanin mRNA levels were strongly increased after lesion of the carotid nerves, both in wild-type and in NGFOE mice. However, whereas NPY mRNA levels decreased in the SCG after axotomy in the wild-type mice, there was a distinct increase in the NGFOE mice. Our results show that high NGF levels in skin induce formation of pericellular baskets in DRGs expressing galanin- and TH-LI and that galanin in these baskets is strongly influenced by peripheral axotomy. However, overexpression of NGF did not markedly influence galanin expression in DRG neurons, neither normally nor after nerve lesions. Finally, expression of NPY in sympathetic ganglia is differently regulated in NGFOE compared to wild-type mice.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-4886
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
290-303
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11161617-Animals, pubmed-meshheading:11161617-Axotomy, pubmed-meshheading:11161617-Galanin, pubmed-meshheading:11161617-Ganglia, Spinal, pubmed-meshheading:11161617-Immunohistochemistry, pubmed-meshheading:11161617-In Situ Hybridization, pubmed-meshheading:11161617-Lumbosacral Region, pubmed-meshheading:11161617-Mice, pubmed-meshheading:11161617-Mice, Inbred C57BL, pubmed-meshheading:11161617-Mice, Transgenic, pubmed-meshheading:11161617-Nerve Growth Factor, pubmed-meshheading:11161617-Neuropeptide Y, pubmed-meshheading:11161617-Neuropeptides, pubmed-meshheading:11161617-Peripheral Nerves, pubmed-meshheading:11161617-RNA, Messenger, pubmed-meshheading:11161617-Sciatic Nerve, pubmed-meshheading:11161617-Skin, pubmed-meshheading:11161617-Superior Cervical Ganglion, pubmed-meshheading:11161617-Sympathectomy, pubmed-meshheading:11161617-Trigeminal Ganglion, pubmed-meshheading:11161617-Tyrosine 3-Monooxygenase
pubmed:year
2001
pubmed:articleTitle
Effect of peripheral nerve lesion and lumbar sympathectomy on peptide regulation in dorsal root ganglia in the NGF-overexpressing mouse.
pubmed:affiliation
Department of Neuroscience, Karolinska Institutet, S-171 77 Stockholm, Sweden. Kristina.Holmberg@neuro.ki.se
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't