Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-2-22
pubmed:databankReference
pubmed:abstractText
Ca2+-binding proteins containing EF-hands are important constituents of intracellular signaling pathways. Recently, three new members of the Neuronal Calcium Sensor subgroup have been cloned in humans. Calsenilin interacts with presenilins, DREAM is a calcium-regulated transcriptional repressor and KChIP3 binds and modulates A-type potassium channels. Here we describe the mouse full-length cDNA and the genomic locus, demonstrating that the three proteins are encoded by the same unique gene. Various mechanisms contribute to the coding potential of this locus. These include alternate translation starts in the first exon and alternative splicing yielding transcripts lacking the EF-hand domains. In situ hybridization, RT-PCR, and Northern blotting reveal nervous system-restricted expression largely coinciding with the distribution of the Kv4.2 alpha-subunit of potassium channels. The presence of transcripts in early embryonic stages suggests roles for the protein also during development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1044-7431
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-16
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed-meshheading:11161465-Alternative Splicing, pubmed-meshheading:11161465-Amino Acid Sequence, pubmed-meshheading:11161465-Animals, pubmed-meshheading:11161465-Base Sequence, pubmed-meshheading:11161465-Blotting, Northern, pubmed-meshheading:11161465-Calcium-Binding Proteins, pubmed-meshheading:11161465-Central Nervous System, pubmed-meshheading:11161465-Cloning, Molecular, pubmed-meshheading:11161465-Codon, Initiator, pubmed-meshheading:11161465-DNA, Complementary, pubmed-meshheading:11161465-Exons, pubmed-meshheading:11161465-In Situ Hybridization, pubmed-meshheading:11161465-Kv Channel-Interacting Proteins, pubmed-meshheading:11161465-Mice, pubmed-meshheading:11161465-Mice, Inbred C57BL, pubmed-meshheading:11161465-Molecular Sequence Data, pubmed-meshheading:11161465-Organ Specificity, pubmed-meshheading:11161465-Potassium Channels, pubmed-meshheading:11161465-Potassium Channels, Voltage-Gated, pubmed-meshheading:11161465-Protein Structure, Tertiary, pubmed-meshheading:11161465-RNA, Messenger, pubmed-meshheading:11161465-Repressor Proteins, pubmed-meshheading:11161465-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11161465-Shal Potassium Channels
pubmed:year
2001
pubmed:articleTitle
Mouse DREAM/calsenilin/KChIP3: gene structure, coding potential, and expression.
pubmed:affiliation
Max-Planck-Institute of Neurobiology, Am Klopferspitz 18A, Martinsried, D-82152, Germany.
pubmed:publicationType
Journal Article