Linked Life Data

11161003

Source:http://linkedlifedata.com/resource/pubmed/id/11161003

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-2-22
pubmed:abstractText
Gene expression profiling of three human temporal lobe brain tissue samples (normal) and four primary glioblastoma multiforme (GBM) tumors using oligonucleotide microarrays was done. Moreover, confirmation of altered expression was performed by whole cell patch clamp, immunohistochemical staining, and RT-PCR. Our results identified several ion and solute transport-related genes, such as N-methyl-d-aspartate (NMDA) receptors, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)-2 receptors, GABA(A) receptor subunits alpha3, beta1, beta2, and beta3, the glutamate transporter, the glutamate/aspartate transporter II, the potassium channel K(V)2.1, hK(V)beta3, and the sodium/proton exchanger 1 (NHE-1), that are all downregulated in the tumors compared with the normal tissues. In contrast, aquaporin-1, possibly aquaporins-3 and -5, and GLUT-3 message appeared upregulated in the tumors. Our results also confirmed previous work showing that osteopontin, nicotinamide N-methyltransferase, murine double minute 2 (MDM2), and epithelin (granulin) are upregulated in GBMs. We also demonstrate for the first time that the cytokine and p53 binding protein, macrophage migration inhibitory factor (MIF), appears upregulated in GBMs. These results indicate that the modulation of ion and solute transport genes and heretofore unsuspected cytokines (i.e., MIF) may have profound implications for brain tumor cell biology and thus may identify potential useful therapeutic targets in GBMs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1531-2267
pubmed:author
pubmed:issnType
Electronic
pubmed:day
7
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21-33
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11161003-Aquaporin 1, pubmed-meshheading:11161003-Aquaporins, pubmed-meshheading:11161003-Blood Group Antigens, pubmed-meshheading:11161003-Brain Neoplasms, pubmed-meshheading:11161003-Gene Expression Profiling, pubmed-meshheading:11161003-Humans, pubmed-meshheading:11161003-Immunohistochemistry, pubmed-meshheading:11161003-Macrophage Migration-Inhibitory Factors, pubmed-meshheading:11161003-Membrane Potentials, pubmed-meshheading:11161003-N-Methylaspartate, pubmed-meshheading:11161003-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:11161003-Patch-Clamp Techniques, pubmed-meshheading:11161003-Potassium Channels, pubmed-meshheading:11161003-RNA, Messenger, pubmed-meshheading:11161003-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:11161003-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11161003-Temporal Lobe
pubmed:year
2001
pubmed:articleTitle
Differential gene expression profiling in human brain tumors.
pubmed:affiliation
Department of Surgery, University of Alabama at Birmingham, Birmingham, Alabama 35294-0005, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't