| pubmed-article:11160902 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11160902 | lifeskim:mentions | umls-concept:C1705597 | lld:lifeskim |
| pubmed-article:11160902 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11160902 | lifeskim:mentions | umls-concept:C0033713 | lld:lifeskim |
| pubmed-article:11160902 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:11160902 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:11160902 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:11160902 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:11160902 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:11160902 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11160902 | pubmed:dateCreated | 2001-2-22 | lld:pubmed |
| pubmed-article:11160902 | pubmed:abstractText | Human c-sis/PDGF-B proto-oncogene has been shown to be overexpressed in a large percentage of human tumor cells establishing a growth-promoting, autocrine growth circuit. Triplex forming oligonucleotides (TFOs) can recognize and bind sequences in duplex DNA, and have received considerable attention because of their potential for targeting specific genomic sites. The c-sis/PDGF-B promoter contains a unique homopurine/homopyrimidine sequence (SIS proximal element, SPE), which is crucial for binding nuclear factors that provoke transcription. In order to develop specific transcriptional inhibitors of the human c-sis/PDGF-B proto-oncogene, 20 potential TFOs targeting part or all of the SPE were screened by gel mobility analysis. DNase I footprinting shows that the TFOs we designed can form a sequence-specific triplex with the target. Protein binding assays demonstrate that triplex formation inhibits nuclear factors binding the c-sis/PDGF-B promoter. Both transient and stable transfection experiments demonstrate that the transcriptional activity of the promoter is considerably inhibited by the TFOs. We propose that TFOs represent a therapeutic potential to specifically diminish the expression of c-sis/PDGF-B proto-oncogene in various pathologic settings where constitutive expression of this gene has been observed. | lld:pubmed |
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| pubmed-article:11160902 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11160902 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11160902 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11160902 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11160902 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11160902 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:11160902 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:11160902 | pubmed:author | pubmed-author:LiuJJ | lld:pubmed |
| pubmed-article:11160902 | pubmed:author | pubmed-author:WangDD | lld:pubmed |
| pubmed-article:11160902 | pubmed:author | pubmed-author:GuoH FHF | lld:pubmed |
| pubmed-article:11160902 | pubmed:author | pubmed-author:JinYY | lld:pubmed |
| pubmed-article:11160902 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:11160902 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:11160902 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:11160902 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11160902 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11160902 | pubmed:pagination | 783-91 | lld:pubmed |
| pubmed-article:11160902 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:11160902 | pubmed:meshHeading | pubmed-meshheading:11160902... | lld:pubmed |
