Source:http://linkedlifedata.com/resource/pubmed/id/11160411
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2001-2-22
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| pubmed:abstractText |
Conflicting data have been collected so far on the action of nitric oxide (NO) on cholinergic interneurons of the striatum. In the present in vitro electrophysiological study, we reported that intracellularly recorded striatal cholinergic interneurons are excited by both hydroxylamine and S-nitroso-N-acetylpenicillamine, two NO donors. This excitation persisted unchanged in the presence of glutamate, dopamine, and substance P receptor antagonists as well as after blockade of tetrodotoxin (TTX)- and calcium channel-sensitive transmitter release, suggesting that NO produces its effects by modulating directly resting ion conductances in the somatodendritic region of striatal cholinergic cells. The depolarizing effect of hydroxylamine was greatly reduced by lowering external concentrations of sodium ions (from 126 to 38 mm) and did not reverse polarity in the voltage range from -120 to -40 mV. The sodium transporter blockers bepridil and 3',4'-dichlorobenzamil were conversely ineffective in preventing NO-induced membrane depolarization. Intracellular cGMP elevation is required for the action of hydroxylamine on striatal cholinergic cells, as demonstrated by the findings that the membrane depolarization produced by this pharmacological agent was prevented by bath and intracellular application of two inhibitors of soluble guanylyl cyclase and was mimicked and occluded by zaprinast, a cGMP phosphodiesterase inhibitor. Finally, intracellular Rp-8-Br-cGMPS, a protein kinase G (PKG) inhibitor, blocked the hydroxylamine-induced membrane depolarization of cholinergic interneurons, whereas both okadaic acid and calyculin A, two protein phosphatase inhibitors, enhanced it, indicating that intracellular PKG and phosphatases oppositely regulate the sensitivity of striatal cholinergic interneurons to NO. The characterization of the cellular mechanisms involved in the regulation of striatal interneuron activity is a key step for the understanding of the role of these cells in striatal microcircuitry.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3',5'-Cyclic-GMP Phosphodiesterases,
http://linkedlifedata.com/resource/pubmed/chemical/8-bromoguanosino-3',5'-cyclic...,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Donors,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1529-2401
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
15
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| pubmed:volume |
21
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1393-400
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:11160411-3',5'-Cyclic-GMP Phosphodiesterases,
pubmed-meshheading:11160411-Acetylcholine,
pubmed-meshheading:11160411-Animals,
pubmed-meshheading:11160411-Corpus Striatum,
pubmed-meshheading:11160411-Cyclic GMP,
pubmed-meshheading:11160411-Cyclic GMP-Dependent Protein Kinases,
pubmed-meshheading:11160411-Electric Stimulation,
pubmed-meshheading:11160411-Enzyme Inhibitors,
pubmed-meshheading:11160411-Excitatory Amino Acid Antagonists,
pubmed-meshheading:11160411-Interneurons,
pubmed-meshheading:11160411-Male,
pubmed-meshheading:11160411-Membrane Potentials,
pubmed-meshheading:11160411-Neurotransmitter Agents,
pubmed-meshheading:11160411-Nitric Oxide,
pubmed-meshheading:11160411-Nitric Oxide Donors,
pubmed-meshheading:11160411-Nitric Oxide Synthase,
pubmed-meshheading:11160411-Patch-Clamp Techniques,
pubmed-meshheading:11160411-Phosphoprotein Phosphatases,
pubmed-meshheading:11160411-Rats,
pubmed-meshheading:11160411-Rats, Wistar,
pubmed-meshheading:11160411-Sodium,
pubmed-meshheading:11160411-Tetrodotoxin,
pubmed-meshheading:11160411-Thionucleotides
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| pubmed:year |
2001
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| pubmed:articleTitle |
Stimulation of nitric oxide-cGMP pathway excites striatal cholinergic interneurons via protein kinase G activation.
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| pubmed:affiliation |
Clinica Neurologica, Dipartimento di Neuroscienze, Università "Tor Vergata," 00133 Rome, Italy.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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