11160378

Source:http://linkedlifedata.com/resource/pubmed/id/11160378

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0002668, umls-concept:C0028128, umls-concept:C0030685, umls-concept:C0039062, umls-concept:C0205227, umls-concept:C0391871, umls-concept:C0443199, umls-concept:C0680255, umls-concept:C0851285, umls-concept:C1283071, umls-concept:C1707723, umls-concept:C1963578, umls-concept:C1979768
pubmed:issue	4
pubmed:dateCreated	2001-2-22
pubmed:abstractText	Nitric oxide (NO) is a potent neuromodulator in the CNS and PNS. At the frog neuromuscular junction (nmj), exogenous application of NO reduces neurotransmitter release, and NO synthases (NOSs), the enzymes producing NO, are present at this synapse. This work aimed at studying the molecular mechanisms by which NO modulates synaptic efficacy at the nmj using electrophysiological recordings and Ca(2+)-imaging techniques. Bath application of the NO donors S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside decreased end plate potential (EPP) amplitude as well as the frequency of miniature EPPs but not their amplitude. Ca(2+) responses elicited in presynaptic terminals by single action potentials were unaffected by NO, but responses evoked by a short train of stimuli were increased. Tonic endogenous production of NO was observed as suggested by the increase in EPP amplitude by bath application of the NO scavenger hemoglobin and the neuronal NOS inhibitor 3-bromo-7-nitroindazole sodium salt. A soluble guanylate cyclase inhibitor, 6-anilino-5,8-quinolinedione (LY-83583), increased EPP amplitude and occluded the effects of the NO donor, suggesting that NO acts via a cGMP-dependent mechanism. High-frequency-induced depression was reduced in the presence of the NO scavenger but not by LY-83583. However, adenosine-induced depression was significantly reduced after bath perfusion of SNAP and in the presence of LY-83583. Our results indicate that NO regulates transmitter release and adenosine-induced depression via a cGMP-dependent mechanism that occurs after Ca(2+) entry and that high-frequency-induced synaptic depression is regulated by NO in a cGMP-independent manner.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/8102140
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Adenosine, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes, http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Cyclase, http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Agents, http://linkedlifedata.com/resource/pubmed/chemical/Nicotinic Antagonists, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Donors, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type I
pubmed:status	MEDLINE
pubmed:month	Feb
pubmed:issn	1529-2401
pubmed:author	pubmed-author:RobitailleRR, pubmed-author:ThomasSS
pubmed:issnType	Electronic
pubmed:day	15
pubmed:volume	21
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	1087-95
pubmed:dateRevised	2006-11-15
pubmed:meshHeading	pubmed-meshheading:11160378-Action Potentials, pubmed-meshheading:11160378-Adenosine, pubmed-meshheading:11160378-Animals, pubmed-meshheading:11160378-Calcium, pubmed-meshheading:11160378-Cyclic GMP, pubmed-meshheading:11160378-Electric Stimulation, pubmed-meshheading:11160378-Enzyme Inhibitors, pubmed-meshheading:11160378-Fluorescent Dyes, pubmed-meshheading:11160378-Guanylate Cyclase, pubmed-meshheading:11160378-Neural Inhibition, pubmed-meshheading:11160378-Neuromuscular Junction, pubmed-meshheading:11160378-Neurotransmitter Agents, pubmed-meshheading:11160378-Nicotinic Antagonists, pubmed-meshheading:11160378-Nitric Oxide, pubmed-meshheading:11160378-Nitric Oxide Donors, pubmed-meshheading:11160378-Nitric Oxide Synthase, pubmed-meshheading:11160378-Nitric Oxide Synthase Type I, pubmed-meshheading:11160378-Rana pipiens, pubmed-meshheading:11160378-Synaptic Transmission
pubmed:year	2001
pubmed:articleTitle	Differential frequency-dependent regulation of transmitter release by endogenous nitric oxide at the amphibian neuromuscular synapse.
pubmed:affiliation	Centre de Recherche en Sciences Neurologiques and Département de physiologie, Université de Montréal, Montréal, Québec, Canada H3C 3J7.
pubmed:publicationType	Journal Article, In Vitro, Research Support, Non-U.S. Gov't