Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-2-22
pubmed:abstractText
The yeast frataxin homolog (Yfh1p) participates in mitochondrial iron homeostasis. The phenotypic defects of the Delta yfh1 mutant include drastic accumulation of iron in mitochondria and slow growth. The Yfh1p precursor protein contains two N-terminal domains that are sequentially cleaved by the matrix processing peptidase on import into mitochondria, generating the mature protein. We have precisely mapped these two cleavage sites. Mutations blocking the first or the second cleavage of Yfh1p do not interfere with its in vitro import or with its ability to complement phenotypes of the Delta yfh1 mutant strain. Distinct roles have been ascertained for the two cleaved domains of Yfh1p. The first cleaved domain (domain I) is sufficient for in vitro mitochondrial import of a non-mitochondrial passenger protein. However, neither domain I nor other matrix-targeting signals alone can support efficient in vitro import of mature Yfh1p. The second cleaved domain (domain II) is required as a spacer between a targeting signal and mature Yfh1p. Likewise, when Yfh1p constructs lacking domain I or II are expressed in vivo, they fail to attain appreciable steady-state amounts in mitochondria and cannot complement phenotypes of the Delta yfh1 mutant.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0964-6906
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
259-69
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11159945-Amino Acid Sequence, pubmed-meshheading:11159945-Binding Sites, pubmed-meshheading:11159945-Biological Transport, pubmed-meshheading:11159945-DNA, Recombinant, pubmed-meshheading:11159945-Genetic Complementation Test, pubmed-meshheading:11159945-Humans, pubmed-meshheading:11159945-Iron-Binding Proteins, pubmed-meshheading:11159945-Metalloendopeptidases, pubmed-meshheading:11159945-Mitochondria, pubmed-meshheading:11159945-Molecular Sequence Data, pubmed-meshheading:11159945-Mutation, pubmed-meshheading:11159945-Phosphotransferases (Alcohol Group Acceptor), pubmed-meshheading:11159945-Plasmids, pubmed-meshheading:11159945-Protein Precursors, pubmed-meshheading:11159945-Protein Processing, Post-Translational, pubmed-meshheading:11159945-Protein Structure, Tertiary, pubmed-meshheading:11159945-Saccharomyces cerevisiae, pubmed-meshheading:11159945-Signal Transduction
pubmed:year
2001
pubmed:articleTitle
Distinct roles for two N-terminal cleaved domains in mitochondrial import of the yeast frataxin homolog, Yfh1p.
pubmed:affiliation
Department of Physiology, University of Pennsylvania School of Medicine, D403 Richards Building, 3700 Hamilton Walk, Philadelphia, PA 19104-6085, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't