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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-2-22
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pubmed:abstractText |
The transcription factor encoded by the mi locus (MITF) is a transcription factor of the basic-helix-loop-helix zipper protein family. Mice of mi/mi genotype express a normal amount of abnormal MITF, whereas mice of tg/tg genotype do not express any MITFs due to the transgene insertional mutation. The effect of normal (+) and mutant (mi) MITFs on the expression of mouse mast cell protease (MMCP) 6 and 7 was examined. Both MMCP-6 and MMCP-7 are tryptases, and their coding regions with high homology are closely located on chromosome 17. Both MMCP-6 and MMCP-7 genes are expressed in normal cultured mast cells (+/+ CMCs). Although the transcription of MMCP-6 gene was severely suppressed in both mi/mi and tg/tg CMCs, that of MMCP-7 gene was severely suppressed only in mi/mi CMCs. The study identified the most significant segment for the transcription in the 5' flanking region of MMCP-7 gene. Unexpectedly, no CANNTG motifs were found that are recognized and bound by +-MITF in this segment. Instead, there was an AP-1 binding motif, and binding of c-Jun to the AP-1 motif significantly enhanced the transcription of MMCP-7 gene. The complex formation of c-Jun with either +-MITF or mi-MITF was demonstrated. The binding of +-MITF to c-Jun enhanced the transactivation of MMCP-7 gene, and that of mi-MITF suppressed the transactivation. Although the former complex was located only in the nucleus, the latter complex was predominantly found in the cytoplasm. The negative effect of mi-MITF on the transcription of MMCP-7 gene appeared to be executed through the interaction with c-Jun.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Mcpt6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Microphthalmia-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitf protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Tpsab1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tryptases
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
645-51
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11157480-Active Transport, Cell Nucleus,
pubmed-meshheading:11157480-Alleles,
pubmed-meshheading:11157480-Animals,
pubmed-meshheading:11157480-Base Sequence,
pubmed-meshheading:11157480-Cell Nucleus,
pubmed-meshheading:11157480-Cells, Cultured,
pubmed-meshheading:11157480-DNA-Binding Proteins,
pubmed-meshheading:11157480-Macromolecular Substances,
pubmed-meshheading:11157480-Mast Cells,
pubmed-meshheading:11157480-Mice,
pubmed-meshheading:11157480-Mice, Inbred C57BL,
pubmed-meshheading:11157480-Mice, Mutant Strains,
pubmed-meshheading:11157480-Microphthalmia-Associated Transcription Factor,
pubmed-meshheading:11157480-Molecular Sequence Data,
pubmed-meshheading:11157480-Promoter Regions, Genetic,
pubmed-meshheading:11157480-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:11157480-RNA, Messenger,
pubmed-meshheading:11157480-Serine Endopeptidases,
pubmed-meshheading:11157480-Transcription Factor AP-1,
pubmed-meshheading:11157480-Transcription Factors,
pubmed-meshheading:11157480-Transcriptional Activation,
pubmed-meshheading:11157480-Tryptases
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pubmed:year |
2001
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pubmed:articleTitle |
Inhibitory effect of the transcription factor encoded by the mutant mi microphthalmia allele on transactivation of mouse mast cell protease 7 gene.
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pubmed:affiliation |
Department of Pathology, Osaka University Medical School, Suita, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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