Linked Life Data

11157074

Source:http://linkedlifedata.com/resource/pubmed/id/11157074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-2-22
pubmed:abstractText
The Golgi complex of skeletal muscle fibers is made of thousands of dispersed elements. The distributions of these elements and of the microtubules they associate with differ in fast compared with slow and in innervated compared with denervated fibers. To investigate the role of muscle impulse activity, we denervated fast extensor digitorum longus (EDL) and slow soleus (SOL) muscles of adult rats and stimulated them directly with patterns that resemble the impulse patterns of normal fast EDL (25 pulses at 150 Hz every 15 min) and slow SOL (200 pulses at 20 Hz every 30 sec) motor units. After 2 weeks of denervation plus stimulation, peripheral and central regions of muscle fibers were examined by immunofluorescence microscopy with regard to density and distribution of Golgi complex, microtubules, glucose transporter GLUT4, centrosomes, and endoplasmic reticulum exit sites. In extrajunctional regions, fast pattern stimulation preserved normal fast characteristics of all markers in EDL type IIB/IIX fibers, although inducing changes toward the fast phenotype in originally slow type I SOL fibers, such as a 1.5-fold decrease of the density of Golgi elements at the fiber surface. Slow pattern stimulation had converse effects such as a 2.2-fold increase of the density of Golgi elements at the EDL fiber surface. In junctional regions, where fast and slow fibers are similar, both stimulation patterns prevented a denervation-induced accumulation of GLUT4. The results indicate that patterns of muscle impulse activity, as normally imposed by motor neurons, play a major role in regulating the organization of Golgi complex and related proteins in the extrajunctional region of muscle fibers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
875-83
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11157074-Animals, pubmed-meshheading:11157074-Electric Stimulation, pubmed-meshheading:11157074-Endoplasmic Reticulum, pubmed-meshheading:11157074-Glucose Transporter Type 4, pubmed-meshheading:11157074-Golgi Apparatus, pubmed-meshheading:11157074-Male, pubmed-meshheading:11157074-Microscopy, Confocal, pubmed-meshheading:11157074-Microtubules, pubmed-meshheading:11157074-Monosaccharide Transport Proteins, pubmed-meshheading:11157074-Muscle, Skeletal, pubmed-meshheading:11157074-Muscle Denervation, pubmed-meshheading:11157074-Muscle Fibers, Fast-Twitch, pubmed-meshheading:11157074-Muscle Fibers, Skeletal, pubmed-meshheading:11157074-Muscle Fibers, Slow-Twitch, pubmed-meshheading:11157074-Muscle Proteins, pubmed-meshheading:11157074-Myosin Heavy Chains, pubmed-meshheading:11157074-Neuromuscular Junction, pubmed-meshheading:11157074-Neuronal Plasticity, pubmed-meshheading:11157074-Rats, pubmed-meshheading:11157074-Rats, Wistar
pubmed:year
2001
pubmed:articleTitle
Golgi complex, endoplasmic reticulum exit sites, and microtubules in skeletal muscle fibers are organized by patterned activity.
pubmed:affiliation
Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-4062, USA. RalstonE@ninds.nih.gov
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't