Source:http://linkedlifedata.com/resource/pubmed/id/11156469
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-1-11
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| pubmed:abstractText |
In the present study, we investigated whether the in vivo bone-forming capacity of human bone marrow-derived stromal cells (HMSCs) could be enhanced by recombinant human bone morphogenetic protein-2 (rhBMP-2). The HMSCs obtained from seven donors (5-54 years of age) were passaged three to six times. Passaged HMSCs exhibited the osteoblastic phenotype in vitro, including: (a) an increase in alkaline phosphatase (ALP) activity in response to dexamethasone, ascorbic acid, and beta-glycerophosphate: and (b) mRNA expression for markers of osteoblastic lineage (ALP, osteopontin, osteocalcin, and parathyroid hormone-receptor) and BMP-2, -4, and -6 detected by reverse transcription-polymerase chain reaction. For the in vivo assay, transplants were subcutaneously implanted into nude mice as follows: group A (vehicle); group B (rhBMP-2); group C (HMSCs with vehicle); and group D (HMSCs with rhBMP-2). Transplants were obtained 2 and 4 weeks after implantation. Correlated radiographic findings, histological observations, and in situ hybridization using species-specific probes showed that the group B transplants contained bone tissue of mouse origin, which was observed at the periphery of the transplants. Four weeks after implantation, small amounts of HMSCs-derived bone tissue were detected at the periphery in two of seven transplants in group C. In contrast, five of seven group D transplants exhibited HMSCs-derived bone tissue, which was located at the center of the transplants and was surrounded by mouse bone tissue. Furthermore, HMSCs-derived chondrogenesis was detected in two of seven group D transplants. The results of the present study demonstrate that culture-expanded HMSCs preserve the osteoblastic phenotype, and the in vivo bone-forming capacity can be promoted by rhBMP-2.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BMP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Bmp2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/recombinant human bone...
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0914-8779
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
19
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
20-8
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:11156469-Adolescent,
pubmed-meshheading:11156469-Adult,
pubmed-meshheading:11156469-Animals,
pubmed-meshheading:11156469-Bone Marrow Cells,
pubmed-meshheading:11156469-Bone Marrow Transplantation,
pubmed-meshheading:11156469-Bone Morphogenetic Protein 2,
pubmed-meshheading:11156469-Bone Morphogenetic Proteins,
pubmed-meshheading:11156469-Cells, Cultured,
pubmed-meshheading:11156469-Child,
pubmed-meshheading:11156469-Child, Preschool,
pubmed-meshheading:11156469-Female,
pubmed-meshheading:11156469-Humans,
pubmed-meshheading:11156469-Male,
pubmed-meshheading:11156469-Mice,
pubmed-meshheading:11156469-Mice, Nude,
pubmed-meshheading:11156469-Middle Aged,
pubmed-meshheading:11156469-Osteoblasts,
pubmed-meshheading:11156469-Osteogenesis,
pubmed-meshheading:11156469-Recombinant Proteins,
pubmed-meshheading:11156469-Stromal Cells,
pubmed-meshheading:11156469-Transforming Growth Factor beta,
pubmed-meshheading:11156469-Transplantation, Heterologous
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| pubmed:year |
2001
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| pubmed:articleTitle |
In vivo bone-forming capacity of human bone marrow-derived stromal cells is stimulated by recombinant human bone morphogenetic protein-2.
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| pubmed:affiliation |
Department of Orthopedic Surgery, Niigata University School of Medicine, Japan.
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| pubmed:publicationType |
Journal Article
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