Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-1-26
pubmed:abstractText
Salicylic acid (SA) is a key endogenous component of local and systemic disease resistance in plants. In this study, we investigated the role of benzoic acid (BA) as precursor of SA biosynthesis in tobacco (Nicotiana tabacum cv Samsun NN) plants undergoing a hypersensitive response following infection with tobacco mosaic virus or in tobacco cell suspensions elicited with beta-megaspermin, an elicitor from Phytophthora megasperma. We found a small pool of conjugated BA in healthy leaves and untreated cell suspensions of tobacco, whereas free BA levels were barely detectable. Infection of plants with tobacco mosaic virus or elicitation of cells led to a rapid de novo synthesis and accumulation of conjugated BA, whereas free BA was weakly induced. In presence of diphenylene iodonium, an inhibitor of superoxide anion formation, SA accumulation was abolished in elicited cells and much higher BA levels were concomitantly induced, mainly as a conjugated form. Furthermore, piperonylic acid, an inhibitor of cinnamate-4-hydroxylase was used as a powerful tool to redirect the metabolic flow from the main phenylpropanoid pathway into the SA biosynthetic branch. Under these conditions, in vivo labeling and radioisotope dilution experiments with [(14)C]trans-cinnamic acid as precursor clearly indicated that the free form of BA produced in elicited tobacco cells is not the major precursor of SA biosynthesis. The main conjugated form of BA accumulating after elicitation of tobacco cells was identified for the first time as benzoyl-glucose. Our data point to the likely role of conjugated forms of BA in SA biosynthesis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-10449575, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-10482671, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-11607555, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-15012523, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-16667674, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-1989492, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-2817901, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-3117032, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-4742778, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-7479795, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-7496401, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-8136368, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-8439298, http://linkedlifedata.com/resource/pubmed/commentcorrection/11154339-9824316
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
318-28
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Free and conjugated benzoic acid in tobacco plants and cell cultures. Induced accumulation upon elicitation of defense responses and role as salicylic acid precursors.
pubmed:affiliation
Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, Université Louis Pasteur, 67084 Strasbourg cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't