|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
11-12
|
|
pubmed:dateCreated |
2001-1-31
|
|
pubmed:abstractText |
The cytoplasmic concentrations of Cl-([Cl-]i) and Ca2+ ([Ca2+]i) were measured with the fluorescent indicators N-(ethoxycarbonylmethyl)-6-methoxyquinilinum bromide (MQAE) and fura-2 in pancreatic beta-cells isolated from ob/ob mice. Steady-state [Cl-]i in unstimulated beta-cells was 34 mM, which is higher than expected from a passive distribution. Increase of the glucose concentration from 3 to 20 mM resulted in an accelerated entry of Cl- into beta-cells depleted of this ion. The exposure to 20 mM glucose did not affect steady-state [Cl-]i either in the absence or presence of furosemide inhibition of Na+, K+, 2 Cl- co-transport. Glucose-induced oscillations of [Ca2+]i were transformed into sustained elevation in the presence of 4,4' diisothiocyanato-dihydrostilbene-2,2'-disulfonic acid (H2DIDS). A similar effect was noted when replacing 25% of extracellular Cl- with the more easily permeating anions SCN-, I-, NO3- or Br-. It is concluded that glucose stimulation of the beta-cells is coupled to an increase in their Cl- permeability and that the oscillatory Ca2+ signalling is critically dependent on transmembrane Cl- fluxes.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/Anions,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Furosemide,
http://linkedlifedata.com/resource/pubmed/chemical/Gallopamil,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/N-(ethoxycarbonylmethyl)-6-methoxyqu...,
http://linkedlifedata.com/resource/pubmed/chemical/Quinolinium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/dihydro-DIDS
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0898-6568
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
12
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
781-6
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11152964-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:11152964-Animals,
pubmed-meshheading:11152964-Anions,
pubmed-meshheading:11152964-Calcium,
pubmed-meshheading:11152964-Calcium Channel Blockers,
pubmed-meshheading:11152964-Calcium Signaling,
pubmed-meshheading:11152964-Cell Membrane Permeability,
pubmed-meshheading:11152964-Cells, Cultured,
pubmed-meshheading:11152964-Chlorides,
pubmed-meshheading:11152964-Electric Conductivity,
pubmed-meshheading:11152964-Fluorescence,
pubmed-meshheading:11152964-Fluorometry,
pubmed-meshheading:11152964-Fura-2,
pubmed-meshheading:11152964-Furosemide,
pubmed-meshheading:11152964-Gallopamil,
pubmed-meshheading:11152964-Glucose,
pubmed-meshheading:11152964-Islets of Langerhans,
pubmed-meshheading:11152964-Mice,
pubmed-meshheading:11152964-Mice, Mutant Strains,
pubmed-meshheading:11152964-Obesity,
pubmed-meshheading:11152964-Quinolinium Compounds
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Microfluorometric analysis of Cl- permeability and its relation to oscillatory Ca2+ signalling in glucose-stimulated pancreatic beta-cells.
|
|
pubmed:affiliation |
Department of Medical Cell Biology, Uppsala University, Biomedicum Box 571, SE-751 23, Uppsala, Sweden.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
