|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2001-1-26
|
|
pubmed:abstractText |
To study the role of extracellular-signal-regulated kinase (ERK) cascade and the small GTP-ase proteins in the activation of the c-fos promoter by angiotensin II (AII), transient transfection experiments were performed in CHO cells stably expressing the rat AT(1A) receptor. In this system AII activated ERK in 1 min and also increased the transcriptional activity of the c-fos promoter-luciferase reporter gene construct. The activation of the promoter proved to be dependent on the Ras-Raf-ERK cascade as cotransfection of expression vectors known to specifically inhibit this cascade blocked the effect of AII. Dominant-negative p21Rac1 mutant partially blocked the activation of the c-fos promoter by AII. However, activation of the c-fos promoter was independent of protein kinase C (PKC) as bisindolylmaleimide I, a specific PKC inhibitor did not block the effect of AII. These results suggest that AII activates the transcription of the c-fos through the Ras-Raf-ERK cascade. Furthermore, p21Rac1 is involved in the modulation of the c-fos promoter by AII.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/rac1 GTP-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/ras Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1018-7782
|
|
pubmed:author |
pubmed-author:AntusBB,
pubmed-author:GoldbergH JHJ,
pubmed-author:HunyadyLL,
pubmed-author:HuszárTT,
pubmed-author:JeneyCC,
pubmed-author:MassziAA,
pubmed-author:MihalikBB,
pubmed-author:MucsiII,
pubmed-author:RosivallLL,
pubmed-author:TerebessyTT,
pubmed-author:ThekkumkaraT JTJ
|
|
pubmed:copyrightInfo |
Copyright 2001 S. Karger AG, Basel
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
9
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
142-9
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11150863-Angiotensin II,
pubmed-meshheading:11150863-Animals,
pubmed-meshheading:11150863-CHO Cells,
pubmed-meshheading:11150863-Cricetinae,
pubmed-meshheading:11150863-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11150863-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11150863-Promoter Regions, Genetic,
pubmed-meshheading:11150863-Protein Kinase C,
pubmed-meshheading:11150863-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:11150863-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:11150863-Receptor, Angiotensin, Type 1,
pubmed-meshheading:11150863-Receptor, Angiotensin, Type 2,
pubmed-meshheading:11150863-Receptors, Angiotensin,
pubmed-meshheading:11150863-Transcription, Genetic,
pubmed-meshheading:11150863-rac1 GTP-Binding Protein,
pubmed-meshheading:11150863-ras Proteins
|
|
pubmed:articleTitle |
Extracellular signal-regulated kinase and the small GTP-binding protein p21Rac1 are involved in the regulation of gene transcription by angiotensin II.
|
|
pubmed:affiliation |
Department of Pathophysiology, Faculty of Medicine, International Nephrology Training and Research Center, Semmelweis University, Budapest, Hungary.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
