|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2001-1-19
|
|
pubmed:abstractText |
Previous research has suggested that cGMP-dependent protein kinases (cGKs) may play a role in long-term potentiation in hippocampus, but their site of action has been unknown. We examined this question at synapses between pairs of hippocampal neurons in dissociated cell culture. Injection of a specific peptide inhibitor of cGK into the presynaptic but not the postsynaptic neuron blocked long-lasting potentiation induced by tetanic stimulation of the presynaptic neuron. As controls, injection of a scrambled peptide or a peptide inhibitor of cAMP-dependent protein kinase into either neuron did not block potentiation. Conversely, injection of the alpha isozyme of cGK type I into the presynaptic but not the postsynaptic neuron produced activity-dependent potentiation that did not require NMDA receptor activation. Evidence from Western blots, reverse transcription-PCR, activity assays, and immunocytochemistry indicates that endogenous cGK type I is present in the neurons, including presynaptic terminals. These results support the idea that cGK plays an important presynaptic role during the induction of long-lasting potentiation in hippocampal neurons.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
1529-2401
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
1
|
|
pubmed:volume |
21
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
143-9
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:11150330-Animals,
pubmed-meshheading:11150330-Antigens, Differentiation,
pubmed-meshheading:11150330-Blotting, Western,
pubmed-meshheading:11150330-Cells, Cultured,
pubmed-meshheading:11150330-Cyclic GMP-Dependent Protein Kinases,
pubmed-meshheading:11150330-Electric Stimulation,
pubmed-meshheading:11150330-Enzyme Inhibitors,
pubmed-meshheading:11150330-Excitatory Amino Acid Antagonists,
pubmed-meshheading:11150330-Excitatory Postsynaptic Potentials,
pubmed-meshheading:11150330-Hippocampus,
pubmed-meshheading:11150330-Immunohistochemistry,
pubmed-meshheading:11150330-Isoenzymes,
pubmed-meshheading:11150330-Long-Term Potentiation,
pubmed-meshheading:11150330-Microinjections,
pubmed-meshheading:11150330-Neurons,
pubmed-meshheading:11150330-Patch-Clamp Techniques,
pubmed-meshheading:11150330-Presynaptic Terminals,
pubmed-meshheading:11150330-Rats,
pubmed-meshheading:11150330-Rats, Sprague-Dawley
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Presynaptic role of cGMP-dependent protein kinase during long-lasting potentiation.
|
|
pubmed:affiliation |
Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
