Source:http://linkedlifedata.com/resource/pubmed/id/11149733
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
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| pubmed:dateCreated |
2001-1-8
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| pubmed:abstractText |
The scid mutation was backcrossed on to the NOD/Shi mouse background, resulting in the development of NOD/Shi-scid mice, which showed lack of mature lymphocytes, macrophage dysfunction and absence of circulating complement, but were not as impaired in natural killer (NK) cell activity as NOD/LtSz-scid mice. We then examined the effect of recipient NK cell depletion by anti-asialo GM1 antiserum on the repopulation of human cord blood (CB) hematopoietic stem cells (HSC) in NOD/Shi-scid mice to clarify the role of recipient NK cells in human HSC engraftment. The anti-asialo GM1 antiserum treatment significantly enhanced the engraftment of CB CD34+ cells, but did not affect the differentiation of the engrafted HSC into each hematopoietic lineage. The NK cell depletion was effective at early stages of the engraftment, but not 3 weeks after the transplantation. The anti-asialo GM1 antiserum treatment did not improve the engraftment by human HSC in scid mice which lack mature lymphocytes, but show neither macrophage dysfunction nor a reduction in circulating complement, indicating that macrophages and/or complement also have roles in HSC graft rejection. The present study indicates that the preconditioning targeting of recipient NK cells in addition to T cell suppression and myeloablation might prevent HSC graft failure, and that NOD/Shi-scid mice treated with anti-asialo GM1 antiserum could provide a useful tool for evaluating the repopulating ability of transplantable human HSC.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Complement System Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/G(M1) Ganglioside,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/asialo GM1 ganglioside
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0268-3369
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1211-6
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11149733-Animals,
pubmed-meshheading:11149733-Antigens, CD34,
pubmed-meshheading:11149733-Cell Differentiation,
pubmed-meshheading:11149733-Complement System Proteins,
pubmed-meshheading:11149733-Fetal Blood,
pubmed-meshheading:11149733-G(M1) Ganglioside,
pubmed-meshheading:11149733-Graft Survival,
pubmed-meshheading:11149733-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:11149733-Hematopoietic Stem Cells,
pubmed-meshheading:11149733-Humans,
pubmed-meshheading:11149733-Immune Sera,
pubmed-meshheading:11149733-Inbreeding,
pubmed-meshheading:11149733-Killer Cells, Natural,
pubmed-meshheading:11149733-Lymphocyte Depletion,
pubmed-meshheading:11149733-Macrophages,
pubmed-meshheading:11149733-Mice,
pubmed-meshheading:11149733-Mice, Inbred NOD,
pubmed-meshheading:11149733-Mice, SCID,
pubmed-meshheading:11149733-Models, Animal,
pubmed-meshheading:11149733-Transplantation, Heterologous,
pubmed-meshheading:11149733-Transplantation Conditioning
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| pubmed:year |
2000
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| pubmed:articleTitle |
Natural killer cell depletion by anti-asialo GM1 antiserum treatment enhances human hematopoietic stem cell engraftment in NOD/Shi-scid mice.
|
| pubmed:affiliation |
Department of Clinical Oncology, The Institute of Medical Science, The University of Tokyo, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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