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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-1-8
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pubmed:abstractText |
Previous studies have shown that calcitonin-like immunoreactive substances are secreted by primary prostate cells. Furthermore, exogenously added calcitonin stimulates proliferation of androgen-responsive LnCaP cells. To examine the possible effect of calcitonin on growth of invasive prostate cancer cells, we tested its effects on proliferation of PC-3M cells. Calcitonin stimulated DNA synthesis of PC-3M cells in a dose-dependent fashion, and also stimulated adenylyl cyclase and protein kinase C activities. To further delineate the role of these signaling cascades in proliferation of PC-3M prostate cancer cells, we selectively activated these pathways by transfecting cDNAs expressing constitutively active forms of either Gsalpha (Gsalpha-QL) or Gqalpha (Gqalpha-QL). cDNAs expressing wild-type forms of G-proteins (Gsalpha-WT and Gqalpha-WT) were used as vehicle controls. Gqalpha-QL transfectants exhibited growth inhibition and terminal differentiation. Those expressing Gsalpha-QL exhibited a dramatic increase in growth rate. Gsalpha-QL transfectants displayed an almost 3-fold increase in [3H]-thymidine incorporation and over a 4-fold increase in growth rate when compared with parental PC-3M cells or those expressing wild-type Gsalpha (Gsalpha-WT). The growth-promoting action of Gsalpha-QL could not be mimicked by either 8-bromo cAMP or forskolin. However, nifedipine, a calcium channel antagonist, potently and selectively inhibited DNA synthesis in Gsalpha-QL transfectants. These results suggest that the growth-promoting actions of Gsalpha on PC-3M cells may be mediated by nifedipine-sensitive proliferative events.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0020-7136
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
46-54
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11149419-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:11149419-Adenylate Cyclase,
pubmed-meshheading:11149419-Blotting, Western,
pubmed-meshheading:11149419-Calcitonin,
pubmed-meshheading:11149419-Calcium Channel Blockers,
pubmed-meshheading:11149419-Calcium Channels,
pubmed-meshheading:11149419-Cell Division,
pubmed-meshheading:11149419-Cyclic AMP,
pubmed-meshheading:11149419-DNA Replication,
pubmed-meshheading:11149419-Dose-Response Relationship, Drug,
pubmed-meshheading:11149419-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11149419-Forskolin,
pubmed-meshheading:11149419-GTP-Binding Protein alpha Subunits, Gs,
pubmed-meshheading:11149419-GTP-Binding Proteins,
pubmed-meshheading:11149419-Humans,
pubmed-meshheading:11149419-Male,
pubmed-meshheading:11149419-Nifedipine,
pubmed-meshheading:11149419-Prostatic Neoplasms,
pubmed-meshheading:11149419-Protein Kinase C,
pubmed-meshheading:11149419-Signal Transduction,
pubmed-meshheading:11149419-Time Factors,
pubmed-meshheading:11149419-Transfection,
pubmed-meshheading:11149419-Tumor Cells, Cultured,
pubmed-meshheading:11149419-Verapamil
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pubmed:year |
2001
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pubmed:articleTitle |
Role of stimulatory guanine nucleotide binding protein (GSalpha) in proliferation of PC-3M prostate cancer cells.
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pubmed:affiliation |
Department of Molecular and Integrative Physiology, The University of Kansas Medical Center, Kansas City, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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