11148131

pubmed:Citation

Pt 2

Surfactant protein C (SP-C) is a lung-specific secreted protein, which is synthesized as a 21-kDa propeptide (SP-C(21)) and then proteolytically processed as a bitopic transmembrane protein in subcellular compartments distal to the medial Golgi to produce a 3.7 kDa mature form. We have shown that initial processing of SP-C(21) involves two endoproteolytic cleavages of the C terminus and that truncation of nine amino acids from the C-flanking peptide resulted in retention of mutant protein in proximal compartments. Because these truncations involved removal of a conserved cysteine residue (Cys(186)), we hypothesized that intralumenal disulfide-mediated folding of the C terminus of SP-C(21) is required for intracellular trafficking. To test this, cDNA constructs encoding heterologous fusion proteins consisting of enhanced green fluorescent protein (EGFP) attached to the N terminus of wild-type rat proSP-C (EGFP/SP-C(1-194)), C-terminally deleted proSP-C (EGFP/SP-C(1-185); EGFP/SP-C(1-191)) or point mutations of conserved cysteine residues (EGFP/SP-C(C122G); EGFP/SP-C(C186G); or EGFP/SP-C(C122/186G)) were transfected into A549 cells. Fluorescence microscopy revealed that transfected EGFP/SP-C(1-194) and EGFP/SP-C(1-191 )were expressed in a punctate pattern within CD-63 positive, EEA-1 negative cytoplasmic vesicles. In contrast, EGFP/SP-C(1-185), EGFP/SP-C(C122G), EGFP/SP-C(C186G) and EGFP/SP-C(C122/186G) were expressed but retained in a juxtanuclear compartment that stained for ubiquitin and that contained (&ggr;)-tubulin and vimentin, consistent with expression in aggresomes. Treatment of cells transfected with mutant proSP-C with the proteasome inhibitor lactacysteine enhanced aggresome formation, which could be blocked by coincubation with nocodazole. Western blots using a GFP antibody detected a single form in lysates of cells transfected with EGFP/SP-C cysteine mutants, without evidence of smaller degradation fragments. We conclude that residues Cys(122) and Cys(186) of proSP-C are required for proper post-translational trafficking. Mutation or deletion of one or both of these residues results in misfolding with mistargeting of unprocessed mutant protein, leading to formation of stable aggregates within aggresomes.

eng

IM

MEDLINE

0021-9533

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NLM

293-302

pubmed-meshheading:11148131-Amino Acid Sequence, pubmed-meshheading:11148131-Cell Line, pubmed-meshheading:11148131-Conserved Sequence, pubmed-meshheading:11148131-Cysteine, pubmed-meshheading:11148131-Cysteine Endopeptidases, pubmed-meshheading:11148131-DNA Primers, pubmed-meshheading:11148131-Green Fluorescent Proteins, pubmed-meshheading:11148131-Humans, pubmed-meshheading:11148131-Luminescent Proteins, pubmed-meshheading:11148131-Lung, pubmed-meshheading:11148131-Multienzyme Complexes, pubmed-meshheading:11148131-Mutagenesis, Site-Directed, pubmed-meshheading:11148131-Peptides, pubmed-meshheading:11148131-Polymerase Chain Reaction, pubmed-meshheading:11148131-Proteasome Endopeptidase Complex, pubmed-meshheading:11148131-Proteolipids, pubmed-meshheading:11148131-Pulmonary Surfactant-Associated Protein C, pubmed-meshheading:11148131-Pulmonary Surfactants, pubmed-meshheading:11148131-Recombinant Fusion Proteins, pubmed-meshheading:11148131-Respiratory Mucosa, pubmed-meshheading:11148131-Sequence Deletion, pubmed-meshheading:11148131-Transfection

Biosynthesis of surfactant protein C: characterization of aggresome formation by EGFP chimeras containing propeptide mutants lacking conserved cysteine residues.

Journal Article, Research Support, U.S. Gov't, P.H.S.