Linked Life Data

11145899

Source:http://linkedlifedata.com/resource/pubmed/id/11145899

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-1-26
pubmed:abstractText
This report focuses mainly on the characterization of a Vero cell line stably expressing the flavivirus Kunjin (KUN) replicon C20SDrep (C20SDrepVero). We showed by immunofluorescence and cryoimmunoelectron microscopy that unique flavivirus-induced membrane structures, termed convoluted membranes/paracrystalline structures, were induced in the C20SDrepVero cells. These induced cytoplasmic foci were immunolabeled with KUN virus anti-NS3 antibodies and with antibodies to the cellular markers ERGIC53 (for the intermediate compartment) and protein disulfide isomerase (for the rough endoplasmic reticulum). However, in contrast to the large perinuclear inclusions observed by immunofluorescence with anti-double-stranded (ds)RNA antibodies in KUN virus-infected cells, the dsRNA in C20SDrepVero cells was localized to small isolated foci scattered throughout the cytoplasm, which were coincident with small foci dual-labeled with the trans-Golgi specific marker GalT. Importantly, persistent expression of the KUN replicons in cells did not produce cytopathic effects, and the morphology of major host organelles (including Golgi, mitochondria, endoplasmic reticulum, and nucleus) was apparently unaffected. The amounts of plus- and minus-sense RNA synthesis in replicon cells were similar to those in KUN virus-infected cells until near the end of the latent period, but subsequently increases of about 10- and fourfold, respectively, occurred in infected cells. Virus-specified protein synthesis in C20SDrepVero cells was also about 10-fold greater than that in infected cells. When several KUN replicon cell lines were compared with respect to membrane induction, the relative efficiencies increased in parallel with increases in viral RNA and protein synthesis, consistent with the increases observed during the virus infectious cycle. Based on these observations, cell lines expressing less-efficient replicons may provide a useful tool to study early events in flavivirus RNA replication, which are difficult to assess in virus infections.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0042-6822
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
161-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11145899-Animals, pubmed-meshheading:11145899-Cell Line, pubmed-meshheading:11145899-Cercopithecus aethiops, pubmed-meshheading:11145899-Cryoelectron Microscopy, pubmed-meshheading:11145899-Encephalitis Viruses, Japanese, pubmed-meshheading:11145899-Fluorescent Antibody Technique, pubmed-meshheading:11145899-Immunohistochemistry, pubmed-meshheading:11145899-Inclusion Bodies, Viral, pubmed-meshheading:11145899-Intracellular Membranes, pubmed-meshheading:11145899-RNA, Double-Stranded, pubmed-meshheading:11145899-RNA, Viral, pubmed-meshheading:11145899-RNA Helicases, pubmed-meshheading:11145899-Replicon, pubmed-meshheading:11145899-Serine Endopeptidases, pubmed-meshheading:11145899-Transfection, pubmed-meshheading:11145899-Vero Cells, pubmed-meshheading:11145899-Viral Nonstructural Proteins, pubmed-meshheading:11145899-Virus Replication
pubmed:year
2001
pubmed:articleTitle
Stable expression of noncytopathic Kunjin replicons simulates both ultrastructural and biochemical characteristics observed during replication of Kunjin virus.
pubmed:affiliation
Clinical Medical Virology Centre, University of Queensland, St. Lucia, Brisbane, Queensland, 4072, Australia. j.mackenzie@mailbox.uq.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't