Source:http://linkedlifedata.com/resource/pubmed/id/11142680
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2001-1-4
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| pubmed:abstractText |
The present study examines putative blastopore determinants in uncleaved Xenopus eggs. Deletion of marginal and lower portions of Xenopus eggs when between 30 and 50% of the first cell cycle has been completed (0.3-0.5 normalized time (NT)) results in the complete absence of the blastopore, while deletion of the vegetal hemisphere during the same period leads to the formation of a smaller blastopore. Extrusion of only yolk and deep cytoplasm of the vegetal hemisphere during 0.3-0.5 NT does not affect the formation or size of the blastopore. Consistently, transplantation of cortical and subcortical cytoplasm from marginal, but not other, sites of eggs at 0.3-0.5 NT to an animal blastomere from 16-cell stage embryos induces an ectopic blastopore and bottle cell-like cells. This does not occur in the same transplantation from eggs at 0.2 NT. These results suggest that the blastopore determinants become localized to the marginal cortical and/or subcortical cytoplasm during 0.2-0.3 NT. Other results suggest the involvement of a hexyleneglycol-sensitive system in the process of localization of the blastopore determinants to the marginal region during 0.2-0.3 NT. The properties and behavior of the putative blastopore determinants are discussed in relation to those of VegT, which previously has been shown to induce ectopic blastopores.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0012-1592
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
42
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
581-91
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading | |
| pubmed:year |
2000
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| pubmed:articleTitle |
Localization and behavior of putative blastopore determinants in the uncleaved Xenopus egg.
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| pubmed:affiliation |
Department of Biology, Faculty of Science, Yamagata University, Japan. shinagaw@sci.kj.yamagata-u.ac.jp
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| pubmed:publicationType |
Journal Article
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