Linked Life Data

11139497

Source:http://linkedlifedata.com/resource/pubmed/id/11139497

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-1-26
pubmed:abstractText
Sexual development in the mushroom Coprinus cinereus is under the control of the A and B mating-type loci, both of which must be different for a compatible, dikaryotic mycelium to form between two parents. The A genes, encoding proteins with homeodomain motifs, regulate conjugate division of the two nuclei from each mating partner and promote the formation of clamp connections. The latter are hyphal configurations required for the maintenance of the nuclear status in the dikaryotic phase of basidiomycetes. The B genes encode pheromones and pheromone receptors. They regulate the cellular fusions that complete clamp connections during growth, as well as the nuclear migration required for dikaryosis. The AmutBmut strain (326) of C. cinereus, in which both A- and B-regulated pathways are constitutively activated by mutations, produces, without mating, dikaryon-like, fertile hyphae with clamp connections. In this study we isolated and characterized clampless1-1 (clp1-1), a mutation that blocks clamp formation, an essential step in A-regulated sexual development, in the AmutBmut background. A genomic DNA fragment that rescues the clp1-1 mutation was identified by transformations. Sequencing of the genomic DNA, together with RACE experiments, identified an ORF interrupted by one intron, encoding a novel protein of 365 amino acids. The clp1-1 mutant allele carries a deletion of four nucleotides, which is predicted to cause elimination of codon 128 and frameshifts thereafter. The clp1 transcript was normally detected only in the presence of the A protein heterodimer formed when homokaryons with compatible A genes were mated. Forced expression of clp1 by promoter replacements induced clamp development without the need for a compatible A gene combination. These results indicate that expression of clp1 is necessary and sufficient for induction of the A-regulated pathway that leads to clamp development.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-10654091, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-1348484, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-1353886, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-1354851, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-1582410, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-1739973, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-3127056, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-3595558, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-3785146, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-5391229, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-7915674, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-8439742, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-9021132, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-9529887, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139497-9671586
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
157
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-40
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
The clp1 gene of the mushroom Coprinus cinereus is essential for A-regulated sexual development.
pubmed:affiliation
Department of Biology, Faculty of Science, Okayama University, Okayama 700-8530, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't