Linked Life Data

11139443

Source:http://linkedlifedata.com/resource/pubmed/id/11139443

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2001-1-26
pubmed:abstractText
Apical ATP, ATP, UTP and UDP evoked transient increases in short circuit current (I(SC), a direct measure of transepithelial ion transport) in confluent Caco-2 cells grown on permeable supports. These responses were mediated by a population of at least three pharmacologically distinct receptors. Experiments using cells grown on glass coverslips showed that ATP and UTP consistently increased intracellular free calcium ([Ca(2+)](i)) whilst sensitivity to UDP was variable. Cross desensitization experiments suggested that the responses to UTP and ATP were mediated by a common receptor population. Messenger RNA transcripts corresponding to the P2Y(2), P2Y(4) and P2Y(6) receptors genes were detected in cells grown on Transwell membranes by the reverse transcriptase - polymerase chain reaction. Identical results were obtained for cells grown on glass. Experiments in which I(SC) and [Ca(2+)](i) were monitored simultaneously in cells on Transwell membranes, confirmed that apical ATP and UTP increased both parameters and showed that the UDP-evoked increase in I(SC) was accompanied by a [Ca(2+)](i)-signal. Ionomycin consistently increased [Ca(2+)](i) in such polarized cells but caused no discernible change in I(SC). However, subsequent application of apical ATP or UTP evoked a small rise in I(SC) but no rise in [Ca(2+)](i). UDP evoked no such response. As well as evoking increases in [Ca(2+)](i), the ATP/UTP-sensitive receptors present in Caco-2 cells thus allow direct control over ion channels in the apical membrane. The UDP-sensitive receptors, however, appear to simply evoke a rise in [Ca(2+)](i).
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-10199813, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-10481222, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-10578132, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-1321817, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-1906749, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-2303453, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-28319, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-3219490, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-7526700, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-7805768, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-8159738, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-8700127, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-8896362, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-9122241, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-9133777, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-9146899, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-9692767, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-9814989, http://linkedlifedata.com/resource/pubmed/commentcorrection/11139443-9886914
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1651-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Multiple P2Y receptor subtypes in the apical membranes of polarized epithelial cells.
pubmed:affiliation
Lung Membrane Transport Group, Tayside Institute of Child Health, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't