Source:http://linkedlifedata.com/resource/pubmed/id/11137219
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
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| pubmed:dateCreated |
2001-1-16
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| pubmed:abstractText |
The nonclassical HLA-G primary transcript is alternatively spliced to generate several mRNAs that have the capacity to encode four membrane bound isoforms, namely HLA-G1, -G2, -G3, and -G4 and two soluble isoforms HLA-G5 and -G6. We aimed at defining the capacity of full length and truncated soluble HLA-G transcripts to be translated in human cell lines. Our study of HLA-G alternative transcripts in various human tissues led us to identify a new splice variant of the HLA-G mRNA, named G7, in which open reading frame continues in intron 2. Due to the presence of a stop codon within intron 2, HLA-G7 transcripts retain the capacity to be translated as soluble truncated HLA-G proteins bearing the alpha1 domain linked to two specific aminoacids encoded by intron 2. Expression vectors containing cDNAs encoding HLA-G5, -G6, and -G7 isoforms were transfected into human cell lines. The presence of translated HLA-G5, -G6, and -G7 proteins was detected in protein extracts of transfected cells by Western blot and immunoprecipitation, but only the full length HLA-G5 soluble isoform could be clearly detected as a secreted protein in both transfected cells supernatants and body fluids.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-G Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0198-8859
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
61
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1138-49
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11137219-Alternative Splicing,
pubmed-meshheading:11137219-Base Sequence,
pubmed-meshheading:11137219-Biopsy,
pubmed-meshheading:11137219-Blotting, Northern,
pubmed-meshheading:11137219-Blotting, Western,
pubmed-meshheading:11137219-Culture Media, Conditioned,
pubmed-meshheading:11137219-DNA, Complementary,
pubmed-meshheading:11137219-HLA Antigens,
pubmed-meshheading:11137219-HLA-G Antigens,
pubmed-meshheading:11137219-Histocompatibility Antigens Class I,
pubmed-meshheading:11137219-Humans,
pubmed-meshheading:11137219-Melanoma,
pubmed-meshheading:11137219-Molecular Sequence Data,
pubmed-meshheading:11137219-Protein Biosynthesis,
pubmed-meshheading:11137219-Protein Isoforms,
pubmed-meshheading:11137219-RNA, Messenger,
pubmed-meshheading:11137219-Transfection,
pubmed-meshheading:11137219-Tumor Cells, Cultured
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| pubmed:year |
2000
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| pubmed:articleTitle |
Identification of HLA-G7 as a new splice variant of the HLA-G mRNA and expression of soluble HLA-G5, -G6, and -G7 transcripts in human transfected cells.
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| pubmed:affiliation |
Service de Recherches en Hémato-Immunologie, CEA/DRM/DSV, Hôpital St. Louis, Institut Universitaire d'Hématologie, Cedex 10, Paris, France. paul@dsvidf.cea.fr
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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