rdf:type |
|
lifeskim:mentions |
umls-concept:C0017596,
umls-concept:C0031809,
umls-concept:C0038128,
umls-concept:C0181839,
umls-concept:C0185115,
umls-concept:C0439851,
umls-concept:C0475363,
umls-concept:C0681504,
umls-concept:C1285573,
umls-concept:C1552596,
umls-concept:C1623640,
umls-concept:C1947931
|
pubmed:issue |
1
|
pubmed:dateCreated |
2001-1-12
|
pubmed:abstractText |
A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.
|
pubmed:commentsCorrections |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0095-1137
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
39
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
401-3
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:11136813-Animals,
pubmed-meshheading:11136813-Cryptosporidiosis,
pubmed-meshheading:11136813-Cryptosporidium parvum,
pubmed-meshheading:11136813-DNA, Protozoan,
pubmed-meshheading:11136813-Feces,
pubmed-meshheading:11136813-Genotype,
pubmed-meshheading:11136813-Humans,
pubmed-meshheading:11136813-Parasitology,
pubmed-meshheading:11136813-Polymerase Chain Reaction,
pubmed-meshheading:11136813-Staining and Labeling
|
pubmed:year |
2001
|
pubmed:articleTitle |
Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination.
|
pubmed:affiliation |
Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT, SE18 8PF, United Kingdom.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|